BIA Separations CIMac Analytical Column AAV 0.1 ml 1.3 um Channels

BIA Separations 110.8503-1.3 CIMac AAV Full/Empty Analytical Column – 0.1 mL, 1.3 µm

The Sartorius BIA Separations 110.8503-1.3 is a premium CIMac Analytical Column engineered to resolve one of the most critical challenges in gene therapy manufacturing: the rapid quantification of empty versus full Adeno-associated virus (AAV) capsids. Built on an advanced polymethacrylate monolithic backbone, this 0.1 mL column features highly interconnected 1.3 µm convective channels. Unlike traditional porous-bead chromatography resins that rely on slow molecular diffusion, this monolithic architecture allows intact viral particles to be transported to the binding sites entirely by convection. This enables extremely high flow rates and rapid analytical run times without sacrificing peak resolution. Functionalized with a highly tuned strong anion exchange (QA) ligand, it effectively resolves the minute charge differences between empty and genome-packed capsids, providing crucial purity and yield data in a matter of minutes.

Technical Performance and Design

• Monolithic Convective Channels: The 1.3 µm channel structure completely eliminates the mass transfer bottlenecks of traditional resins, allowing for extremely rapid volumetric flow rates and high-throughput analytical screening.

• Strong Anion Exchange (AEX) Chemistry: The finely tuned quaternary amine ligand provides the critical selectivity required to resolve the minimal isoelectric point (pI) differences between empty capsids and full viral payloads.

• Flow-Independent Resolution: Maintains sharp chromatographic peak profiles and distinct baseline separation even at elevated flow rates, yielding highly accurate quantitation of impurities, aggregates, and intact vectors.

• Universal System Compatibility: Features standard 10-32 UNF coned ports designed to interface seamlessly with 1/16-inch OD tubing on any modern bio-inert HPLC or UHPLC system.

Product Specifications

• Catalog Number: 110.8503-1.3

• Column Type: Analytical Monolith (CIMac)

• Target Application: AAV Full/Empty Capsid Separation and Quantitation

• Bed Volume: 0.1 mL

• Channel Size: 1.3 µm

• Ligand Type: Strong Anion Exchanger (QA modified for AAV)

• Maximum Operating Pressure: 15 MPa (150 bar / 2175 psi)

Key Benefits & Applications

• Accelerates Process Development: Serves as a rapid, real-time Process Analytical Technology (PAT) tool, providing immediate feedback on capsid ratios during upstream cell culture harvests and downstream purification.

• Wide Serotype Flexibility: Analytically validated to successfully bind, separate, and elute a broad spectrum of standard AAV serotypes currently utilized in clinical gene therapy pipelines.

• Superior Analytical Precision: Yields highly reproducible quantitative data, allowing research scientists to strictly monitor batch-to-batch consistency of critical clinical viral vector products.

• Robust Reusability: The rugged poly(glycidyl methacrylate-co-ethylene dimethacrylate) backbone is highly stable, withstanding repeated analytical injections and standard clean-in-place (CIP) protocols without degrading.

All product names, logos, and brands are property of their respective owners. BIA Separations is a Sartorius brand.

Used. In excellent condition.