Biotage Microplate Isolute SLE Plus 96 Well 400 mg Each Well Sealed Plate

Biotage 820-0400-P01 ISOLUTE SLE+ 96-Well Plate – 400 µL Capacity

The Biotage 820-0400-P01 is an ISOLUTE SLE+ (Supported Liquid Extraction) 96-well plate engineered as a high-throughput, automatable alternative to traditional liquid-liquid extraction (LLE). Each well contains a highly processed, inert diatomaceous earth support optimized to hold up to 400 µL of aqueous sample (often referred to by its 400 mg sorbent mass). When an aqueous biological fluid is loaded, it spreads thinly over the sorbent, maximizing the contact surface area. A water-immiscible organic extraction solvent is then applied, efficiently desorbing target analytes directly into a collection plate. This efficient "load-wait-elute" mechanism prevents the formation of sample emulsions and produces exceptionally clean extracts entirely free of proteins and phospholipids.

Technical Performance and Design

• Emulsion-Free Extraction: Because the aqueous sample is immobilized on the solid support before the organic solvent is introduced, the two liquid phases never physically mix, entirely preventing the formation of frustrating, yield-robbing emulsions.

• Rapid Load-Wait-Elute Protocol: Replaces the tedious shaking, capping, and centrifugation steps of traditional LLE. Researchers simply load the sample, wait five minutes for absorption, and elute with an organic solvent.

• Diatomaceous Earth Sorbent: The highly homogenous support media ensures consistent, reproducible flow characteristics from well to well, preventing blockages and ensuring uniform recovery rates across all 96 samples.

• Fixed 96-Well Architecture: The standardized footprint interfaces seamlessly with automated liquid handlers, vacuum manifolds, and positive pressure systems (such as the Biotage PRESSURE+ 96) for rapid, parallel sample processing.

Product Specifications

• Catalog Number: 820-0400-P01

• Product Line: ISOLUTE SLE+ (Supported Liquid Extraction)

• Format: 96-Well Fixed Plate

• Sorbent Type: Diatomaceous Earth (Inert Support)

• Loading Capacity: 400 µL (Aqueous Sample) / 400 mg Sorbent Mass

• Extraction Mechanism: Phase Partitioning (Solid-Supported Liquid-Liquid Extraction)

Key Benefits & Applications

• Bioanalytical & Clinical Testing: Essential for extracting pharmaceutical drugs, steroid panels, and fat-soluble vitamins from complex, viscous human fluids like plasma, whole blood, and urine prior to LC-MS/MS analysis.

• Maximized Extract Purity: By excluding large matrix components like proteins and phospholipids, the SLE+ plate drastically reduces mass spectrometer ion suppression and protects the instrument source from fouling.

• Enhanced Throughput: Cuts 96-well sample preparation time essentially in half compared to manual LLE methods, enabling clinical labs to process heavy daily testing queues efficiently.

• High Recovery Rates: Frequently delivers analyte recoveries exceeding 90% with outstanding run-to-run reproducibility (RSD < 10%).

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