Harvard Apparatus Filter Microplate DispoDialyzers 96 Well 10 kDa MWCO

Harvard Apparatus 74-0903 96-Well DispoDialyzer – 10 kDa MWCO

The Harvard Apparatus 74-0903 is a 96-Well DispoDialyzer designed for the simultaneous, high-throughput dialysis of small-volume biological samples. Each well is equipped with a premium regenerated cellulose membrane featuring a strict 10,000 Dalton Molecular Weight Cut-Off (10 kDa MWCO). Accommodating individual sample volumes from 25 µL to 300 µL, this single-use microplate format allows researchers to rapidly perform buffer exchange, desalting, or detergent removal on up to 96 discrete samples in parallel. The plate is engineered to float directly on the surface of the dialysis buffer, maximizing the diffusion surface area and drastically accelerating clearance times compared to traditional sealed tubing.

Technical Performance and Design

• Individual Well Membranes: Each of the 96 wells features its own distinct regenerated cellulose membrane. This physical isolation completely eliminates the risk of well-to-well cross-contamination or fluid bridging during the diffusion process.

• Direct-Float Architecture: The buoyant polymer plate frame allows the entire 96-well assembly to float securely on the dialysis buffer without requiring cumbersome external flotation collars or buoys.

• Secure Strip-Cap Sealing: The system includes twelve 8-cap strips that press firmly into the plate, providing a leak-proof seal that prevents sample evaporation and protects against buffer ingress during rigorous stirring.

• High Volumetric Recovery: The inert regenerated cellulose and optimized vertical well geometry prevent macromolecule entrapment, routinely providing quantitative recovery rates of ≥95% for dilute proteins and oligonucleotides.

Product Specifications

• Catalog Number: 74-0903

• Format: 96-Well Microplate Dialyzer

• Volume Capacity: 25 µL to 300 µL per well

• Membrane Material: Regenerated Cellulose

• Molecular Weight Cut-Off (MWCO): 10 kDa (10,000 Daltons)

• Packaging: Pack of 2 Plates (Includes 8-cap sealing strips)

Key Benefits & Applications

• High-Throughput Desalting: The optimal format for parallel buffer exchange or the rapid removal of urea, imidazole, and high salt concentrations from up to 96 purified protein fractions simultaneously.

• LC-MS Sample Preparation: Efficiently dialyzes away small molecular weight detergents, unreacted crosslinkers, and unbound labeling dyes prior to high-performance liquid chromatography or mass spectrometry.

• Workflow Automation: The standard 96-well spacing allows for rapid, simultaneous sample loading using 8-channel or 12-channel pipettes and automated liquid handlers.

• Trace Metal Free: The premium cellulose membranes are pre-cleaned and manufactured free of heavy metal contaminants and sulfur, preserving the biological activity of sensitive enzymes and active pharmaceutical ingredients.

All product names, logos, and brands are property of their respective owners.