Novasep Filter Cassette SIUS Prostream Low Binding PES 0.1 m2 300 kD

Novasep / Sartorius PP300M01L SIUS™ Prostream 300 kD TFF Cassette (0.1 m²)

The Novasep PP300M01L is a premium single-use Tangential Flow Filtration (TFF) cassette designed for the rapid concentration and diafiltration of high-value biological products. Featuring the Prostream low-binding Polyethersulfone (PES) membrane, this unit is engineered to minimize protein-membrane interactions, ensuring exceptionally high product recovery and consistent flux. The 300 kD Molecular Weight Cut-Off (MWCO) is optimized for processing large biomolecules, vaccines, and viral vectors. As part of the SIUS™ family, this cassette is delivered pre-sanitized and ready for immediate use, allowing for a "plug-and-play" workflow that eliminates the need for complex cleaning validation (CIP) and cross-contamination concerns in multi-product facilities.

Technical Performance and Design

• Prostream Low-Binding PES: The modified membrane surface is designed to be highly hydrophilic, reducing non-specific binding of proteins and ensuring that even sensitive or "sticky" biologics can be processed with maximum yield.

• Single-Use (SIUS™) Efficiency: Specifically designed to be disposed of after a single batch, this cassette removes the burden of cleaning validation and the associated chemical/water costs, significantly accelerating the transition from setup to production.

• Optimized Fluid Dynamics: The internal flow path is engineered to provide uniform transmembrane pressure (TMP) across the 0.1 m² surface area, preventing localized fouling and maintaining a high, stable flux throughout the run.

• Predictable Scale-Up: The Prostream membrane chemistry and SIUS™ hardware geometry are consistent across all sizes, enabling seamless linear scale-up from this 0.1 m² development unit to full-scale manufacturing modules.

Product Specifications

• Catalog Number: PP300M01L

• Membrane Type: Prostream (Low-Binding Polyethersulfone / PES)

• MWCO: 300 kD

• Filtration Area: 0.1 m² (1.1 ft²)

• Format: SIUS™ Plate-and-Frame Cassette

• Condition: Delivered pre-sanitized in a vacuum-sealed bag.

• Regulatory Compliance: Manufactured in an ISO-certified facility; Materials meet USP Class VI biological reactivity standards.

Key Benefits & Applications

• Viral Vector Concentration: The 300 kD pore size is the preferred specification for the concentration and buffer exchange of AAV, Lentivirus, and other gene therapy vectors.

• Vaccine Production: Ideal for the purification of virus-like particles (VLPs) and large antigens where high recovery and process speed are critical to maintaining product stability.

• Multi-Product Facilities: The single-use nature of the SIUS™ system makes it perfect for contract manufacturing organizations (CMOs) where eliminating the risk of batch-to-batch cross-contamination is paramount.

• R&D and Pilot Studies: Provides a cost-effective solution for small-batch processing and process development without the capital expenditure for permanent TFF cleaning infrastructure.

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