Repligen MidiKros Hollow Fiber Filter mPES 300 kD

Repligen MicroKros® C04-E300-05-N Hollow Fiber Filter (300 kD, mPES)

The Repligen C04-E300-05-N is a high-precision MicroKros® Tangential Flow Filtration (TFF) module designed for the gentle processing of low-volume biological samples. Utilizing a 300 kD Molecular Weight Cut-Off (MWCO) modified Polyethersulfone (mPES) membrane, this filter is engineered for the efficient concentration and diafiltration of large biomolecules, virus-like particles (VLPs), and nanoparticles. The 0.5 mm fiber ID provides a balance of low shear stress and high flux, ensuring maximum viability for sensitive samples. With a 40 cm² effective surface area and a 41.5 cm effective length, this MicroKros unit is the industry standard for researchers performing small-scale TFF in the 1 ml to 20 ml range.

Technical Performance and Design

• Modified Polyethersulfone (mPES) Membrane: The ProConnex® mPES chemistry is inherently hydrophilic, resulting in exceptionally low non-specific binding and high product recovery compared to traditional PES or RC membranes.

• MicroKros® Small-Volume Format: Optimized for laboratory-scale R&D, these modules minimize "dead volume" and holdup, making them ideal for high-value proteins and limited-quantity biological samples.

• Gentle Tangential Flow: The hollow fiber geometry provides a low-shear environment that protects the structural integrity of large proteins, antibodies, and viral vectors during recirculation.

• Plug-and-Play Integration: Featuring standard Luer-lock connections, this module is compatible with manual syringe-based TFF or automated KrosFlo® systems for controlled, reproducible results.

Product Specifications

• Catalog Number: C04-E300-05-N

• Membrane Type: modified Polyethersulfone (mPES)

• MWCO: 300 kD

• Fiber ID: 0.5 mm

• Effective Filtration Area (EFA): 40 cm²

• Effective Length: 41.5 cm

• Connections: Female Luer-Lock (Inlet/Outlet and Permeate ports)

• Purity: Non-pyrogenic; Manufactured in a controlled environment.

Key Benefits & Applications

• Virus & VLP Concentration: The 300 kD pore size is specifically optimized for retaining viral particles and large macromolecular complexes while allowing smaller contaminants and salts to pass into the permeate.

• Nanoparticle Desalting: Ideal for cleaning and concentrating drug-delivery nanoparticles and liposomes where maintaining particle size distribution is critical.

• Antibody/Protein Diafiltration: Provides a fast and efficient alternative to dialysis for buffer exchange in small-volume protein samples, reducing process time from days to hours.

• Scalability: The mPES membrane and fiber path are identical to larger Repligen TFF modules, ensuring that process parameters developed on this MicroKros unit can be linearly scaled to production-level KrosFlo® systems.

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