Agilent HPLC Column 10 um Non-Porous 4.6 x 250 mm

The Agilent 5190-2455 HPLC Column is a specialized high-performance liquid chromatography (HPLC) column designed for biopharmaceutical applications, particularly for the analysis of charge variants of monoclonal antibodies (MAbs), proteins, and peptides. This column features a 10 µm non-porous particle size and has dimensions of 4.6 mm internal diameter by 250 mm in length.

The core of this column is its Bio WCX (Weak Cation Exchange) stationary phase. This phase consists of a weak cation exchange layer chemically bonded to the hydrophilic polymer coating of the non-porous particles. Non-porous particles are highly advantageous in bioseparations because they minimize diffusion distances for large molecules, leading to exceptionally high efficiency and resolution, crucial for resolving complex charge variants. The non-porous nature ensures efficient mass transfer and high resolution separations, while the weak cation exchange functionality allows for charge-based separations of biomolecules.

The column hardware is made from PEEK (Polyether Ether Ketone), which is a biocompatible and bio-inert material. This PEEK construction ensures that the column does not interact with sensitive biomolecules, preventing non-specific binding and maintaining the integrity of the sample, making it suitable for coupling with bio-compatible LC systems. The Agilent 5190-2455 HPLC Column provides a robust solution for charge variant analysis in biopharma, offering high resolution and reliable performance for comprehensive characterization of therapeutic proteins.The Agilent 5190-2455 HPLC Column is a specialized high-performance liquid chromatography (HPLC) column designed for biopharmaceutical applications, particularly for the analysis of charge variants of monoclonal antibodies (MAbs), proteins, and peptides. This column features a 10 µm non-porous particle size and has dimensions of 4.6 mm internal diameter by 250 mm in length.

The core of this column is its Bio WCX (Weak Cation Exchange) stationary phase. This phase consists of a weak cation exchange layer chemically bonded to the hydrophilic polymer coating of the non-porous particles. Non-porous particles are highly advantageous in bioseparations because they minimize diffusion distances for large molecules, leading to exceptionally high efficiency and resolution, crucial for resolving complex charge variants. This allows for excellent peak symmetries and minimizes protein loss. The weak cation exchange functionality enables charge-based separations of biomolecules.

The column hardware is made from PEEK (Polyether Ether Ketone), which is a biocompatible and bio-inert material. This PEEK construction ensures that the column does not interact with sensitive biomolecules, preventing non-specific binding and maintaining the integrity of the sample. This makes it ideally suited for coupling with bio-compatible or bio-inert LC systems. The Agilent 5190-2455 HPLC Column provides a robust solution for charge variant analysis in biopharma, offering high resolution and reliable performance for comprehensive characterization of therapeutic proteins.