Macherey-Nagel NucleoSpin Filters Mini Columns Total of 100 Columns

Macherey-Nagel NucleoSpin Filters Mini Columns – Polypropylene Filtration Columns with Plastic Washers, Cleanroom Quality (MPN: 740606.50 / 740606.250)

The Macherey-Nagel 740606 NucleoSpin Filters (cataloged under base MPN 740606, including 740606.50 for 50 prep units and 740606.250 for 250 prep units) are specialized polypropylene mini filtration spin columns designed for rapid, debris-free sample clarification prior to nucleic acid or protein isolation. Unlike standard silica-membrane DNA/RNA binding columns, these filter columns contain a high-porosity polyethylene filter matrix engineered to remove cell debris, un-lysed tissue fragments, precipitate, and viscous genomic DNA from crude biological lysates. Used primarily as a pre-filtration step in RNA extractions, plant sample preps, and viscous tissue homogenations, the NucleoSpin Filters ensure smooth flow-through, prevent downstream silica-membrane clogging, and improve overall nucleic acid purity and yield.

Technical Performance and Engineering Design

• High-Efficiency Lysate Clarification: Traps cellular remnants, insoluble debris, and high-molecular-weight sticky aggregates in a single rapid centrifugation step, eliminating the need for tedious manual syringe filtration or prolonged high-speed pelleting.

• Prevention of Downstream Column Clogging: Clearing sticky precipitates and viscous tissue debris prior to binding prevents flow blockage on downstream silica mini-spin columns, guaranteeing optimal flow-through rates and high-purity nucleic acid recovery.

• Chemical-Inert Polypropylene & PE Construction: Molded from high-purity, low-binding virgin polypropylene equipped with an inert polyethylene filter frit to prevent non-specific analyte binding or chemical leaching into sensitive lysates.

• Universal Centrifuge Compatibility: Standard mini-spin column format fits perfectly into standard 1.5 mL / 2.0 mL microcentrifuge rotors and vacuum manifolds (such as Macherey-Nagel NucleoVac 96 or standard lab manifolds).

Product Specifications

• Part Number / MPN: 740606 (Available as 740606.50 [50-pack] or 740606.250 [250-pack])

• Manufacturer / Brand: Macherey-Nagel GmbH & Co. KG

• Component Type: Pre-Filtration Mini Spin Columns (Filter Frits, non-binding)

• Sample Volume Capacity: Up to 750 microliters (µL) per spin column

• Filter Material: Porous Polyethylene (PE) matrix

• Housing Material: Medical-Grade Virgin Polypropylene (PP)

• Format Interoperability: Standard 2.0 mL collection tube footprint; compatible with microcentrifuges operating up to 11,000 x g

Key Benefits and Applications

• Plant & Fungal RNA / DNA Isolation: Essential for filtering polysaccharide-rich, fibrous, or tough plant tissue homogenates (e.g., maize, pine needles, or yeast samples) prior to silica column binding.

• Viscous Lysate Pre-Clearing: Rapidly reduces viscosity caused by dense genomic DNA or mucous proteins in complex animal tissue, stool, or soil extracts.

• Upstream Cleanup for NucleoSpin Kits: Serves as direct replacement filter columns for Macherey-Nagel NucleoSpin RNA, NucleoSpin Plant II, and custom molecular isolation protocols.

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