Varian SPEC C18 Microplate 96 Well 30 mg

Varian / Agilent Bond Elut SPEC C18 96-Round-Well Solid Phase Extraction (SPE) Microplate – 30 mg Sorbent Bed, 1 mL Well Capacity, Monolithic Disk Technology (MPN: A5960330)

The Varian A5960330 (now cataloged as Agilent Bond Elut SPEC C18 / C18AR, MPN A5960330) is a high-throughput 96-well Solid Phase Extraction (SPE) microplate engineered for rapid, clean, and highly reproducible sample preparation in liquid chromatography-mass spectrometry (LC-MS/MS) and bioanalytical workflows. Featuring a 30 mg C18 octadecylsilane sorbent bed per well, this plate incorporates SPEC micro-disk technology, which encapsulates the C18 stationary phase within a monolithic fiberglass matrix. This design eliminates particle channeling, prevents silica fining, and drastically reduces void volume compared to traditional loose-packed SPE cartridges. Engineered with a standard 1 mL well volume, the A5960330 enables ultra-low elution volumes for maximum sample concentration, high analyte recovery, and minimal solvent consumption in demanding pharmaceutical, clinical, and environmental extraction protocols.

Technical Performance and Monolithic Disk Engineering

• Monolithic Glass Fiber Micro-Disk Technology: Integrates the C18 sorbent into a uniform, porous fiberglass disk rather than loose particle beds. This eliminates particle channeling and bed drying issues, ensuring consistent flow rates and uniform extraction kinetics across all 96 wells.

• Ultra-Low Elution Volume Capability: The minimal void volume of the 30 mg SPEC disk allows analytes to be eluted in significantly smaller solvent volumes (as low as 100 to 200 microliters). This reduces or eliminates time-consuming evaporation and reconstitution steps prior to LC-MS/MS injection.

• High-Purity Octadecylsilane (C18) Chemistry: Retains non-polar organic compounds, pharmaceuticals, metabolites, and biomolecules from aqueous biological matrices through hydrophobic reversed-phase interactions.

• Automation-Friendly Microplate Format: Molded from ultra-clean polypropylene to conform to standard ANSI/SLAS 96-well footprints, ensuring full compatibility with automated liquid handlers, robotic workstations, and positive pressure or vacuum manifold extraction manifolds.

Product Specifications

• Part Number / MPN: A5960330 (Cross-listed as Varian A5960330 / Agilent Bond Elut SPEC C18 A5960330)

• Manufacturer / Brand: Varian, Inc. / Agilent Technologies

• Microplate Format Array: 96 Round-Well Matrix

• Sorbent Type / Chemistry: C18 (Octadecylsilane) Reversed-Phase Monolithic Disk

• Sorbent Mass: 30 mg per well

• Individual Well Volume: 1.0 mL total volume per well

• Housing Material: Medical-grade, ultra-clean Polypropylene (PP)

• System Interoperability: Compatible with Agilent Positive Pressure Manifold 96 (PPM-96), vacuum manifolds, and standard 96-well collection plates

Key Benefits and Applications

• Bioanalytical & Pharmacokinetic Sample Prep: Ideal for extracting drug candidates, metabolites, steroids, and small molecules from plasma, serum, urine, and whole blood prior to quantitative LC-MS/MS analysis.

• High-Throughput Method Conversion: Enables seamless transition from traditional 1 mL or 3 mL C18 SPE cartridges to a high-density 96-well automated workflow without sacrificing retention capacity or clean extract purity.

• Reduced Solvent Consumption & Processing Time: Fast flow-through and low elution requirements reduce overall solvent costs, waste disposal fees, and total analytical turnaround time.

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