Corning Microplate 24 Well PS Total of 20 Plates

Corning BioCoat 354414 Poly-D-Lysine Multiwell Plate – 24-Well, Clear, Flat Bottom

The Corning BioCoat 354414 is a 24-well cell culture microplate engineered to enhance the adhesion, spreading, and survival of fastidious or transfected cell lines. Molded from optically clear virgin polystyrene, the flat-bottom wells are uniformly pre-coated with Poly-D-Lysine (PDL), a synthetic, positively charged molecule that alters surface charge to improve protein absorption and cellular attachment. Supplied sterile and complete with a matching lid, this assay-ready plate eliminates the variability of in-house coating protocols and is optimized for serum-free or reduced-serum cultures, primary neuron survival, and neurite outgrowth assays.

Technical Performance and Design

• Poly-D-Lysine (PDL) Surface: The uniform application of synthetic PDL (MW 75-150 kD) creates a positively charged matrix that promotes firm attachment of fastidious cells without stimulating unwanted biological activity or introducing natural polymer impurities.

• Crystalline Optical Clarity: Manufactured from premium clear polystyrene with perfectly flat bottoms, providing excellent light transmission for high-resolution microscopy and automated bottom-reading imaging systems.

• Standardized Culture Footprint: Each well provides a 2.0 cm² growth area and an approximate 1.0 mL functional working volume, adhering strictly to standard microplate dimensions for universal lab integration.

• Contamination Control: Includes a dedicated, one-way fitting lid that facilitates stable gas exchange while protecting the wells from ambient particulate contamination and evaporative media loss.

Product Specifications

• Catalog Number: 354414

• Format: 24-Well Plate with Lid

• Material: Clear Polystyrene (PS)

• Well Geometry: Round Wells, Flat Bottom

• Growth Area: 2.0 cm² per well

• Surface Coating: Poly-D-Lysine (PDL)

• Sterility: Sterile, Non-Pyrogenic

Key Benefits & Applications

• Primary Neuronal Culture: The gold standard surface for supporting the differentiation, neurite outgrowth, and extended survival of sensitive central nervous system (CNS) primary cells.

• Transfected Cell Lines: Drastically improves the anchoring and monolayer consistency of fastidious or weakly adherent transfected cells (such as HEK-293 or PC12 lines) during rigorous washing steps.

• Assay-Ready Consistency: Eliminates tedious manual laboratory coating procedures, ensuring highly reproducible lot-to-lot surface chemistry for quantitative bioassays.

• Serum-Free Cultivation: Provides the baseline electrostatic support necessary for cells to anchor and proliferate in defined, serum-free or reduced-serum media environments.

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