Greiner Bio-One Culture Microplate w Lid 96 Well Sealed Case of 32 Plates

Greiner Bio-One CELLSTAR 96-Well Black/µClear® Tissue Culture Microplate (655090) – Case of 32

The Greiner Bio-One 655090 is a highly specialized, optical-bottom 96-well microplate engineered for demanding cell-based fluorescence, luminescence, and high-content screening (HCS) assays. Part of the renowned CELLSTAR® series, these plates feature an opaque black polystyrene matrix to eliminate cross-talk, seamlessly fused to a ultra-flat, paper-thin µClear® film bottom for exceptional transmission down to the UV spectrum. Provided pre-sterilized with a dedicated condensation-ring lid in a sealed factory case of 32 plates, this tissue culture (TC) treated array delivers the reliable surface chemistry required for uniform adherent cell monolayer expansion and automated microscopic analysis.

Technical Performance & Design

• Opaque Black Matrix with µClear® Bottom: Constructed with solid black pigments in the well sidewalls to achieve an absolute light barrier, effectively eliminating well-to-well optical cross-talk during highly sensitive fluorescence and luminescence assays.
• Vacuum-Gas Plasma TC Surface Treatment: Subjected to a proprietary physical surface modification process that adds hydrophilic polar groups directly onto the polystyrene matrix, establishing optimal cell attachment and spreading kinetics.
• Individual Chimney Well Geometry: Designed using an isolated chimney well profile where each cylindrical well is separated by a small air gap, completely preventing accidental capillary fluid bridges and liquid cross-contamination during manual or robotic additions.
• Evaporation-Reducing Condensation Ring Lid: Outfitted with a high-transparency polystyrene lid containing custom-profiled internal condensation rings that map perfectly over each well to reduce evaporation while maintaining unrestricted gas exchange layers.

Product Specifications

• Model Number / Part No: 655090 (CELLSTAR® / µClear® Assay Line)
• Manufacturer: Greiner Bio-One GmbH
• Plate Configuration: 96-Well Array (Standard 8 x 12 Grid Matrix)
• Well Shape & Bottom: Round Chimney Wells with an Ultra-Flat Film Bottom
• Color Morphology: Solid Black Frame with a Clear Optical Well Base
• Total Well Capacity: 392 µL per Well (Recommended working volume: 25 µL to 340 µL)
• Sterility Specification: Sterile (Pre-sterilized via validated beta irradiation)
• Purity Verification: Certified free from detectable RNase, DNase, human DNA, and endotoxins (Non-pyrogenic)
• Packaging Configuration: Case of 32 Plates (Packaged as 8 plates per pack, 4 packs per case)

Key Benefits & Applications

• Fluorescence and Luminescence Screening: Specifically optimized for top-reading or bottom-reading optical instruments, yielding high signal-to-noise ratios and low background autofluescence.
• High-Magnification Live-Cell Imaging: The ultra-thin film bottom mirrors standard cover glass specifications, allowing microscopic objectives to easily approach the cell plane for clean focal resolution under phase-contrast or confocal systems.
• Standardized Automation Handling: Adheres strictly to universal ANSI/SLAS footprint layouts, ensuring reliable gripping, stacking, and scanning pathways across all modern microplate automated instrumentation lines.
• Alphanumeric Matrix Indexing: Outfitted with clear, high-contrast molded alphanumeric margin markers alongside a distinct bevel-cut corner to guarantee precise sample orientation and eliminate micro-volume mapping errors.

Greiner Bio-One®, CELLSTAR®, and µClear® are registered trademarks of Greiner Bio-One GmbH. The 655090 catalog code indicates the black/clear bottom, TC-treated variant with a lid. If your analytical workflows require an entirely solid black format or non-treated surfaces for suspension cultures, separate catalog numbers must be specified.