Thermo Scientific Storage Bottle 250 ml Polystyrene Case of 24 Bottles

Thermo Scientific Nalgene Rapid-Flow Sterile Filter Storage Bottle – 250 mL, Clear Polystyrene, 45 mm Neck (MPN: 455-0250)

The Thermo Scientific Nalgene 455-0250 is a premium, 250 mL sterilizing-grade filter receiver and storage bottle engineered for mammalian tissue culture, media preparation, and biological fluid archiving. Molded from ultra-pure, crystal-clear virgin polystyrene (PS), this bottle is designed to function seamlessly as a replacement receptacle for Nalgene Rapid-Flow™ bottle-top vacuum filters or as an independent, single-use fluid storage container. Equipped with an ergonomic, white high-density polyethylene (HDPE) 45 mm screw cap, the assembly establishes a certified leak-proof physical seal that completely prevents evaporation and protects pH levels. Every bottle features a generous 20% internal headspace allowance to accommodate downstream additions of serum, growth factors, or enzyme supplements without overflowing the vessel brim.

Technical Performance and Design

• Rapid-Flow System Integration: Features a precision-molded 45 mm neck diameter that screws directly onto Nalgene bottle-top filters. This allows it to handle immediate downstream vacuum forces without structural collapse, cracking, or thread slipping.

• Optically Clear Virgin Polystyrene: Fabricated from crystal-grade, low-extractable polystyrene that provides absolute glass-like transparency for effortless visual inspection of fluid clarity, colorimetric changes, and precipitate formations.

• Hermetic Seal Integrity: The 1½-turn threaded closure system utilizes a premium virgin HDPE screw cap. This design establishes an airtight mechanical barrier that halts gas transport, preventing ambient $CO_2$ tracking and long-term media pH shifts.

• Ergonomic Hand Grips: The structural silhouette is designed with pronounced tapered sides and molded structural grips, allowing for slip-free manipulation and steady pouring when handled inside laminar flow hoods with damp nitrile gloves.

Product Specifications

• Catalog Number / MPN: 455-0250 (4550250)

• Manufacturer / Brand: Thermo Scientific / Nalgene™ Rapid-Flow™

• Volumetric Capacity: 250 mL (8 oz.)

• Bottle Material: Virgin Clear Polystyrene (PS)

• Cap Material / Size: Virgin High-Density Polyethylene (HDPE) / 45 mm

• Neck Finish Configuration: 45 mm Threaded Screw Mouth

• Sterility Status: Gamma Irradiated Sterile (Individually Bagged, 5-Year Sterile Shelf Life)

• Purity Certifications: Certified Non-Pyrogenic, Non-Cytotoxic, and low-extractable standard

Key Benefits & Applications

• Tissue Culture Media Preparation: The gold standard vessel for receiving, collecting, and storing freshly filtered cell culture media, nutrient broths, and volatile biological buffers.

• Bypassed Cross-Contamination: The single-use, sterile packaging layout completely eliminates laboratory overhead costs, multi-hour hot autoclaving protocols, and cross-contamination risks associated with washing reusable glass bottles.

• Integrated Additive Headspace: Explicitly engineered to hold more than its 250 mL nominal volume, giving researchers a safety buffer to comfortably add fetal bovine serum (FBS), antibiotics, or amino acids to the pre-filtered broth base.

• Dual-Sided High-Contrast Graduations: Imprinted with high-contrast, permanent structural volumetric scale lines on two sides, allowing for precise tracking of both filling metrics and residual pouring volumes.