Fisherbrand Microplate 96 Well PCR Plate Polypropylene 0.3ml Natural 50 Plates

Fisherbrand 14230244 Semi-Skirted PCR Plate – 96-Well, Natural Polypropylene, 300 µL

The Fisherbrand 14230244 (also written as 14-230-244) is a standard-profile 96-well microplate engineered for demanding thermal cycling, real-time quantitative PCR (qPCR), and downstream genetic sequencing. Molded from premium, bio-inert virgin polypropylene under strict cleanroom conditions, this plate features clear well bottoms that provide high sample visibility. The semi-skirted geometry adds essential structural rigidity, preventing the plate deck from warping during extreme thermal expansion. Additionally, the plate incorporates a convenient cut-away corner alongside a high-contrast alphanumeric grid for rapid sample tracking and mistake-free loading.

Technical Performance and Design

• Semi-Skirted Stability: The surrounding perimeter semi-skirt adds structural reinforcement compared to unskirted options, ensuring the plate remains perfectly flat for seamless interface with automated robotic handlers, plate stackers, and barcode label machines.

• Raised-Rim Well Architecture: Each individual well opening is engineered with a pronounced, raised rim. This design concentrates vertical compression force to establish a tight, leak-proof perimeter seal when paired with optical adhesive films, cap strips, or heat-sealing foils.

• Direct ABI/Sequencer Compatibility: The physical dimensions allow it to drop directly into standard Applied Biosystems (ABI) thermal cyclers and 96-well capillary sequencers without requiring specialized structural adapters or deck shims.

• Pure Virgin Polypropylene: Manufactured completely free of synthetic lubricants, heavy metals, or biocides. This safeguards sensitive master mixes from chemical leaching that can cause baseline drift or inhibit polymerase activity.

Product Specifications

• Catalog Number / MPN: 14230244 (14-230-244)

• Product Line: Fisherbrand 96-Well PCR Plates

• Format: 96-Well Plate (8 × 12 Array)

• Plate Profile: Standard Profile

• Skirt Style: Semi-Skirted

• Color / Tint: Natural / Clear Wells

• Maximum Working Volume: 300 µL (When used with heat or adhesive seals)

• Purity Metrics: Cleanroom produced, certified free of detectable RNase, DNase, and human DNA

Key Benefits & Applications

• High-Throughput PCR & qPCR: Provides uniform wall thickness across all 96 wells, accelerating thermal transfer velocities and reducing well-to-well variance for quantitative data generation.

• Sanger Sequencing Preparation: Widely used as a reliable baseline vessel for running amplification steps and big-dye purification sequences on high-throughput sequencing decks.

• Evaporative Protection: The combination of uniform plastic wells and high raised rims minimizes liquid volume depletion, preventing sample drying during long, high-temperature denaturation blocks.

• Robotic Liquid Handling: High mechanical deck rigidity prevents the plate from bowing under downforce, eliminating missed wells or tip crashes from automated liquid handling heads.

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