Thermo Scientific Slide-A-Lyzer Cassette 10K 0.5 ml Box of 10 Cassettes

The Thermo Scientific 66383 Slide-A-Lyzer Cassette is a specialized laboratory tool designed for the efficient dialysis and purification of small-volume biological samples. This cassette has a small sample capacity of 0.5 ml (500 µl), making it suitable for processing precious or limited solutions.

A key feature of this cassette is its 10K (10,000 Dalton or 10 kDa) molecular weight cutoff (MWCO). This means the semipermeable membrane is designed to retain molecules with a mass greater than 10 kDa (such as proteins or large nucleic acids) while allowing smaller molecules, like salts, buffers, and small metabolites, to pass freely into the surrounding dialysis buffer. The Slide-A-Lyzer design simplifies the dialysis process by eliminating the need for traditional dialysis tubing and providing a convenient, leak-proof system with a built-in air bubble barrier.

This cassette is a vital tool in biochemistry and molecular biology for applications such as protein concentration, buffer exchange, and desalting prior to final analysis or purification.