Corning Terrific Broth 1 L Case of 6 Bottles

Corning 46-055-CM Terrific Broth Liquid Medium – 1 L Bottle

The Corning 46-055-CM is a premium, ready-to-use 1 L liquid microbiological growth medium engineered explicitly for the high-density propagation and maintenance of recombinant Escherichia coli strains. Formulated under strict ISO 13485 quality standards, Terrific Broth (TB) is an enriched nutrient matrix containing higher concentrations of casein peptone and yeastolate than standard Luria-Bertani (LB) broths. It is supplemented with glycerol as a non-repressible carbohydrate carbon source and features an integrated potassium phosphate buffering system. This chemical architecture sustains prolonged bacterial logarithmic growth phases, yielding significantly higher cell masses and dramatically maximizing plasmid DNA or heterologous protein recovery during downstream nucleic acid purification workflows.

Technical Performance and Design

• Enriched Yeastolate & Peptone Matrix: Formulated with 24.00 g/L Yeastolate and 12.00 g/L Casein Peptone. This high concentration of amino acids, vitamins, and trace elements supplies the structural baseline necessary to support highly dense bacterial biomasses.

• Non-Repressible Glycerol Carbon Source: Utilizes 4.00 mL/L Glycerol rather than glucose as the primary carbohydrate source. This prevents the rapid accumulation of acetic acid byproducts—a common cause of premature cell death and culture crash in glucose-supplemented mediums.

• Phosphate Buffering Stabilization: Incorporates a balanced mixture of $2.31\text{ g/L KH}_2\text{PO}_4$ (monopotassium phosphate) and $12.54\text{ g/L K}_2\text{HPO}_4$ (dipotassium phosphate). This system maintains physiological pH bounds against acid production, allowing cultures to thrive far beyond standard saturation thresholds.

• Ready-to-Use (1X) Form: Pre-mixed, sterile-filtered, and autoclaved inside a heavy-duty polystyrene storage bottle. This removes the lab variability, dust exposure, and preparation labor associated with compounding dehydrated powder media in-house.

Product Specifications

• Catalog Number / MPN: 46-055-CM (46055CM)

• Manufacturer / Brand: Corning® / Mediatech

• Volume Capacity: 1 Liter (1 L)

• Media Type: Terrific Broth (Liquid, 1X Form)

• Formulation: 12.00 g Casein Peptone, 24.00 g Yeastolate, 4.00 mL Glycerol, 2.31 g $\text{KH}_2\text{PO}_4$, 12.54 g $\text{K}_2\text{HPO}_4$ per Liter

• Storage Profile: Ambient storage between 15°C to 30°C

• Sterility Matrix: Sterile-Filtered & Autoclavable, Lot-Validated Certificate of Analysis (CoA) Compliant

Key Benefits & Applications

• Maximized Plasmid Yields: The premier chosen liquid medium for expanding E. coli cultures in preparation for high-yield plasmid minipreps, midipreps, and maxipreps.

• Recombinant Protein Expression: Supports massive target protein yields during IPTG- or auto-induction sequences, minimizing the total culture volumes required to collect adequate protein fractions.

• High Cell Density Cultivation: Achieves up to a 4-to-10-fold increase in bacterial biomass and cell saturation profiles compared to basic Luria-Bertani (LB) broth formulations.

• Validated Manufacturing: Produced within certified cGMP facilities, ensuring highly reproducible lot-to-lot chemical metrics for regulated academic and industrial biotechnology pipelines.

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