Macherey-Nagel NucleoTrap PCR Clean-up Pack of 10 Preps

Macherey-Nagel 740584.10 NucleoTrap DNA Purification Kit – 10 Preps, Gel Extraction & PCR Clean-Up

The Macherey-Nagel 740584.10 is a specialized 10-prep molecular biology kit engineered for the highly efficient purification and desalt cleaning of DNA fragments from agarose gels and direct PCR reaction mixtures. Utilizing a column-free, liquid-phase silica matrix suspension technology, this kit eliminates the fluid constraints and structural limitations of standard microcentrifuge spin columns. The activated silica bead matrix binds target DNA fragments ranging from 20 bp up to approximately 50 kbp under highly concentrated chaotropic salt conditions. Following rapid ethanol-based wash cycles to strip away primers, enzymes, dNTPs, and salts, pure genomic or plasmid templates are eluted in a highly concentrated, low-ionic-strength format ready for downstream application.

Technical Performance and Design

• Column-Free Silica Suspension: Relies on a pipettable, premium silica bead matrix instead of physical membrane columns. This flexible format allows researchers to scale the matrix volume proportionally to the target DNA quantity, optimizing micro-elutions down to 20 µL without structural dead-volume losses.

• Chaotropic Binding System: Supplied with specialized binding buffers (Buffer NT1 and Buffer NT2) infused with high-density chaotropic salts. Buffer NT1 incorporates targeted properties designed to rapidly liquefy standard and low-melting TAE or TBE agarose gel slices safely up to 200 mg.

• Expansive Fragment Cutoff Range: Provides an exceptionally broad binding retention window, capturing ultra-small fragments starting down at 20 bp all the way up to massive 50 kbp structural plasmids, ensuring full recovery of non-standard nucleic acid lines.

• Rapid 45-to-60 Minute Protocol: The simple batch-centrifugation sequence operates swiftly under standard benchtop configurations, processing multiple individual preps simultaneously to bypass complex vacuum manifolds or specialized magnetic racks.

Product Specifications

• Catalog Number / MPN: 740584.10

• Manufacturer: Macherey-Nagel

• Product Family: NucleoTrap® System

• Kit Size Capacity: 10 Preparations (10 Preps)

• Technology Format: Silica Bead Suspension / Matrix Fluid Phase

• Fragment Size Window: 20 bp to approximately 50 kbp

• Typical Fluid Recovery Yield: 50% to 90% (Dependent on base fragment size)

• Downstream Safety: Eluted fragments are fully compatible with Next-Gen Sequencing (NGS), automated cloning, Sanger sequencing, restriction digestion, and real-time qPCR mapping.

Kit Components Included

• NucleoTrap® Suspension (100 µL): Activated silica bead matrix.

• Binding Buffer NT1 (6 mL): Optimized agarose gel solubilizer.

• Binding Buffer NT2 (10 mL): Secondary chaotropic environment driver.

• Wash Buffer NT3 Concentrate (6 mL): Requires the addition of 24 mL of 96–100% ethanol prior to first run.

• Elution Buffer NE (13 mL): 5 mM Tris-HCl (pH 8.5) matrix solution.

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