Millipore Column SeQuant ZIC-HILIC 5um 150 x 21.2 mm with Warranty

The Millipore SeQuant ZIC-HILIC column is a specialized high-performance liquid chromatography (HPLC) column designed to retain and separate polar and hydrophilic compounds. This particular column has a particle size of 5 µm and dimensions of 150 mm in length by 21.2 mm in internal diameter, making it a semi-preparative column suitable for scaling up methods from analytical to production.

The column's stationary phase is a silica-based sorbent with a covalently bonded, highly polar, permanent zwitterion. This unique chemistry enables hydrophilic interaction liquid chromatography (HILIC), a separation technique that uses a mobile phase with a high concentration of an organic solvent. The elution order in HILIC is the reverse of reversed-phase chromatography, as more polar compounds are retained more strongly, which makes the ZIC-HILIC column an ideal tool for separating compounds that would otherwise elute in the void volume on reversed-phase columns. This includes compounds such as carbohydrates, metabolites, acids, bases, and amino acids.

The zwitterionic stationary phase offers robust performance with high selectivity and reproducibility. It is also well-suited for LC-MS applications, as the high organic content in the mobile phase can enhance analyte ionization and increase sensitivity. The FDA has even recommended it for the analysis of melamine and related contaminants. The large dimensions of this column make it ideal for purifying larger quantities of material.