Eppendorf BioBLU Bioreactor 3c Sterile 5 L Single Use

Eppendorf BioBLU 3c Single-Use Bioreactor – 5 L, Macrosparger, Pitched-Blade Impeller, Sterile

The Eppendorf 1386000300 BioBLU 3c is a premier single-use bioreactor designed to provide the performance of a traditional stirred-tank vessel with the convenience and safety of disposable technology. This 5-liter rigid-wall vessel is specifically optimized for mammalian cell culture, featuring a single pitched-blade impeller that provides gentle, low-shear mixing essential for sensitive cell lines such as CHO, HEK293, and stem cells. The macrosparger configuration is ideal for high-density cultures where efficient gassing is required with minimal foaming. Supplied fully sterile and pre-assembled with non-invasive optical sensors, the BioBLU 3c eliminates the need for cleaning, autoclaving, and probe insertion, significantly reducing the risk of contamination and increasing laboratory throughput.

Technical Performance and Design

• Rigid-Wall Stirred-Tank Design: Combines the proven geometry of glass bioreactors with the benefits of single-use materials, ensuring easy scale-up and consistent mixing dynamics across the BioBLU portfolio.

• Macrosparger Gassing: Features a macrosparger for submerged aeration, providing excellent mass transfer while reducing the small-bubble shear stress and foaming often associated with microspargers.

• Non-Invasive Optical Sensors: Pre-installed optical "spots" for pH and Dissolved Oxygen (DO) allow for real-time monitoring through the vessel wall, eliminating the contamination risks associated with traditional probe insertion.

• Magnetic Overhead Drive: Utilizing a sealed magnetic coupling for the impeller, this design ensures a complete microbial barrier and maintains vessel integrity throughout long-term cultures.

Product Specifications

• Catalog Number: 1386000300

• Total Volume: 5.0 L

• Working Volume Range: 1.25 L – 3.75 L

• Impeller Type: 1 x Pitched-Blade Impeller (Down-flow)

• Sparger Type: Macrosparger

• Material: USP Class VI Polycarbonate (Animal Component-Free)

• Sterility: Sterile (Irradiated, SAL 10⁻⁶)

Key Benefits & Applications

• Bioprocess Scale-Up: Geometrically similar to the BioBLU 0.3c and 50c models, making it a critical tool for bridging the gap between small-scale R&D and pilot-scale production.

• Risk Mitigation: The single-use nature eliminates cross-contamination between runs and the need for complex cleaning validation (CIP/SIP) procedures.

• Cell and Gene Therapy: Optimized for high-value suspension cultures where sterile integrity and low-shear environments are non-negotiable for maintaining cell viability and titer.

• Controller Compatibility: Seamlessly integrates with Eppendorf BioFlo 120 and BioFlo 320 control stations, as well as various legacy and third-party systems via available adapter kits.

Expiration: 2024-02.

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