Ambion Centrifuge Tube 0.5 ml Snap Cap Sterile Case of 1000 Tubes

Ambion Nonstick RNase-Free Microcentrifuge Tubes - 0.5 mL, Low-Binding Polypropylene, Clear, Graduated, Flat Snap Cap (MPN: AM12300)

The Ambion AM12300 (manufactured under the Invitrogen / Thermo Fisher Scientific portfolio) is a premium, molecular-biology-grade microcentrifuge tube engineered for handling ultra-low volumes of sensitive nucleic acids, proteins, and genomic materials. Molded from high-purity clear polypropylene, these 0.5 mL tubes feature a specialized, ultra-hydrophobic "nonstick" surface treatment that drastically reduces non-specific binding. This surface chemistry achieves exceptional recovery rates for rare target sequences or concentrated enzymes without introducing chemical lubricant slip-agents that could leach into down-stream assays. Every tube includes an attached, flat snap cap that forms a tight mechanical seal capable of withstanding severe thermal stresses without bursting open.

Technical Performance and Design

• Guaranteed Bio-Purity Profile: Extensively validated and guaranteed to be entirely free of detectable RNase and DNase enzymes. This pristine surface makes them immediately ready for high-stakes transcriptomics and molecular extraction lines right out of the bulk packaging.

• Leach-Free Nonstick Surface Chemistry: Utilizing a specialized low-binding polymer modification rather than silicone or chemical lubricants, these tubes prevent valuable proteins or DNA from adhering to the container walls while safeguarding the liquid mixture from experimental artifacts.

• Boil-Proof Positive Seal Cap: The integrated flat snap-cap features an engineered positive-lock rim that maintains structural integrity across a vast temperature spectrum (from minus 90 C to plus 121 C). This profile establishes a positive seal design that prevents caps from opening during high-temperature boiling water baths or prolonged centrifugal stresses.

• High-Contrast Graduation Metrics: Marked with graduations for convenience across the clear conical side-profile, allowing for rapid, accurate visual checks of micro-aliquots during serial dilutions.

Product Specifications

• Catalog Number / MPN: AM12300

• Manufacturer / Brand: Thermo Fisher Scientific / Ambion (Invitrogen Product Line)

• Volumetric Capacity: 0.5 mL (500 microliters)

• Surface Coating: Nonstick, Low-Binding Matrix

• Material Composition: Pure Virgin Polypropylene (PP)

• Closure Type: Attached Flat Snap Cap (Flat lids for easy labeling / Matte Zone)

• Thermal Performance Horizon: Stable from ultra-low freezer storage at minus 90 C up to autoclaving at 121 C

• Sterility Status: Non-Sterile but certified fully RNase/DNase-free and completely autoclave-safe

Key Benefits and Applications

• RNA Extraction and Storage: The baseline industry standard for processing, isolating, and archiving highly sensitive mRNA, miRNA, or total RNA extracts where ambient ribonuclease degradation must be aggressively blocked.

• Next-Generation Sequencing (NGS): Extensively deployed in genomics core facilities for preparing low-volume sequencing libraries, holding expensive enzyme master mixes, and distributing fluorometric quantification standards (e.g., Qubit assays).

• Dilute Protein and Peptide Handling: The low-binding polypropylene chemistry eliminates sample hang-up, preventing rare antibodies or dilute peptide hormones from trapping on the lower conical walls.

• Flat Cap Labeling Optimization: The completely smooth, flat surface top of the snap-cap includes a frosted texture zone, facilitating clean, smear-resistant marking with standard laboratory cryogenic pens.

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