Celltreat Microplate 12 Wells Case of 50 Culture Microplates with Lids

Celltreat 12-Well Cell Culture Plate – Clear Polystyrene, Flat Bottom, with Lid, Tissue Culture Treated, Sterile (MPN: 229111)

The Celltreat 229111 is a premium, sterile 12-well tissue culture plate engineered for consistent cell attachment, spreading, and growth of anchorage-dependent cell lines. Molded from ultra-pure, crystal-grade USP Class VI virgin polystyrene, this standard-format plate features an 3 × 4 well array with a generous growth area of 3.85 cm² per well. Each well undergoes an advanced vacuum gas-plasma surface treatment, rendering the hydrophobic plastic surface permanently hydrophilic and optimal for robust cell monolayer formation. Shipped individually wrapped in medical-grade paper/plastic peel-to-open pouches with a matching clear, condensation-ringed lid, this plate provides reliable performance for multi-well experimental setups, cloning expansions, and scale-up routines.

Technical Performance and Design

• Tissue Culture (TC) Treated Surface Chemistry: Every plate undergoes a validated vacuum gas-plasma treatment to incorporate oxygen-containing functional groups into the polystyrene backbone. This yields a highly uniform, hydrophilic surface that optimizes cell anchoring, spreading, and proliferation metrics.

• Optically Clear Flat Bottom Base: Features a perfectly flat well bottom profile (F-bottom style) with a working volume capacity up to 6.8 mL. This design provides an ultra-consistent focal plane across the culture area, supporting artifact-free high-magnification phase-contrast microscopy and bottom-read plate readers.

• Evaporation-Reducing Lid with Condensation Rings: Supplied with a matching transparent polystyrene lid designed with built-in condensation rings. This layout aligns directly over individual wells to guide moisture drop-back, minimizing cross-well contamination, cross-talk, and peripheral edge-effect evaporation.

• Aseptic Individual Peel-Pack Sterility: Processed via validated gamma irradiation to achieve a 10⁻⁶ Sterility Assurance Level (SAL). Every plate is individually sealed in a medical-grade paper/plastic wrapper and certified non-pyrogenic, non-cytotoxic, and DNase/RNase-free.

Product Specifications

• Catalog Number / MPN: 229111

• Manufacturer / Brand: Celltreat® Scientific Products

• Format: Standard 12-Well Matrix (3 × 4 Layout)

• Growth Area Per Well: 3.85 cm²

• Total Well Volume Capacity: 6.8 mL

• Well Bottom Profile: Flat Bottom (F-Well)

• Material Composition: Crystal-Clear USP Class VI Virgin Polystyrene (PS)

• Sterility Status: Gamma Irradiated Sterile (SAL 10⁻⁶)

• Compliance Standard: Fully conforms to standard ANSI/SLAS microplate dimensional footprints

Key Benefits & Applications

• Cell Expansion & Transfection Scale-Up: The standard choice for expanding adherent cell lines before harvesting, or conducting transfections, viral infections, and staining protocols across multiple variable conditions.

• Colorimetric Assay Optimization: High-purity optical properties support precise data acquisition during standard multi-well colorimetric tests, cell viability screenings (MTT/XTT), and dynamic macrophage activation protocols.

• High-Contrast Sample Mapping: Features bold, raised alphanumeric grid markings (A–C, 1–4) next to each well, providing rapid visual orientation and mistake-free plate indexing inside biosafety cabinets.

• Robotic Handing Compliance: Rigidly engineered outer dimensions ensure smooth, error-free automated platform integration with robotic gripper arms, multi-well liquid handlers, automated imagers, and plate stackers.

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Expiration: 2022-08-18.