Cytiva 28966367 Accessory Kit HiScale 16

Cytiva 28966367 Accessory Kit for HiScale™ 16 Chromatography Columns

The Cytiva 28966367 is a comprehensive accessory and maintenance kit specifically designed for the HiScale™ 16 line of high-performance chromatography columns. This kit provides the critical consumable components required to maintain the structural integrity and fluid dynamic efficiency of the column. It includes precision-molded O-rings, specialized net-fasteners, and essential connection fittings that ensure a leak-proof seal under high-pressure conditions (up to 20 bar). By utilizing genuine Cytiva replacement parts, researchers can ensure consistent flow distribution across the resin bed, preventing channeling and maintaining peak resolution during complex protein purification and fractionation workflows.

Technical Performance and Design

• Precision Pressure Sealing: The included O-rings are manufactured from high-grade elastomers designed to withstand the mechanical stress of pressurized chromatography and the chemical rigors of common FPLC buffers.

• Optimized Flow Distribution: The net-fasteners are engineered to hold the column bed supports (frits) securely in place, ensuring a uniform mobile phase front across the 16 mm column diameter for maximum separation efficiency.

• Chemical Compatibility: All components are validated for use with standard aqueous buffers, high-salt solutions, and common cleaning-in-place (CIP) agents such as 1M NaOH and 20% Ethanol.

• Seamless System Integration: Features standard M6 and 1/16" connectors (depending on the specific kit revision) for rapid, tool-free connection to ÄKTA™ systems and other FPLC platforms.

Product Specifications

• Catalog Number: 28-9663-67

• Compatibility: Cytiva HiScale™ 16/20 and HiScale™ 16/40 columns.

• Kit Contents: O-rings (various sizes), Net-fasteners, and tubing connectors.

• Material: PEEK, Ethylene Propylene (EPDM), and Polypropylene components.

• Maximum Pressure: Validated for use up to 2 MPa (20 bar / 290 psi).

Key Benefits & Applications

• Routine Column Maintenance: Essential for the annual or bi-annual "overhaul" of HiScale columns to prevent accidental leaks that could result in the loss of high-value purified proteins.

• Bio-Process Development: Ideal for researchers scaling up from Tricorn™ or HiTrap™ columns to larger HiScale™ formats, ensuring the hardware is configured for maximum performance.

• Contamination Control: Regularly replacing O-rings and fasteners prevents the buildup of microbial biofilms or residual proteins in the dead spaces of the column hardware.

• ÄKTA™ Workflow Optimization: Guaranteed compatibility with the ÄKTA™ avant, ÄKTA™ pure, and ÄKTA™ start systems for a streamlined chromatography experience.

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