Cytiva 28966375 Accessory Kit HIScale 50

Cytiva 28966375 Accessory Kit for HiScale™ 50 Chromatography Columns

The Cytiva 28966375 is a specialized maintenance and connection kit engineered for the HiScale™ 50 line of high-performance chromatography columns. This kit provides the critical consumable components required to maintain a secure, high-pressure seal and optimal fluid dynamics for 50 mm diameter column hardware. It includes precision-molded O-rings and specialized net-fasteners designed to withstand the mechanical stresses of pressurized chromatography (up to 20 bar). By utilizing genuine Cytiva replacement parts, laboratory professionals can ensure uniform flow distribution across the resin bed, effectively preventing channeling and protecting high-value biological samples during large-scale protein purification and fractionation.

Technical Performance and Design

• Large-Format Pressure Sealing: The included O-rings are specifically sized for the 50 mm HiScale series, manufactured from high-grade elastomers that maintain their integrity across a wide range of temperatures and FPLC buffer conditions.

• Enhanced Flow Distribution: The net-fasteners are designed to provide even tension across the column bed supports, ensuring a stable and uniform mobile phase front across the entire 50 mm cross-section.

• Superior Chemical Compatibility: All components are validated for use with standard aqueous buffers, high-salt solutions, and common sanitization agents, including 1M NaOH and 20% Ethanol.

• Tool-Free Integration: Specifically engineered for the HiScale™ 50/20 and 50/40 column formats, allowing for rapid maintenance and seamless connectivity with ÄKTA™ avant, ÄKTA™ pure, and ÄKTA™ pilot systems.

Product Specifications

• Catalog Number: 28966375 (formerly 28-9663-75)

• Compatibility: Cytiva HiScale™ 50 mm Diameter Columns

• Kit Contents: Precision O-rings, Net-fasteners, and Support Screen components.

• Maximum Pressure: Validated for use up to 2 MPa (20 bar / 290 psi).

• Materials: PEEK, EPDM, and Polypropylene for robust chemical resistance.

Key Benefits & Applications

• Large-Scale Purification: Essential for maintaining the hardware used in pilot-scale and large-volume protein purification where hardware failure could result in significant product loss.

• Column Overhaul & Refurbishment: The primary kit for the routine replacement of seals and fasteners to ensure the column meets original manufacturer performance specifications.

• Aseptic Process Integrity: Regularly replacing these components prevents the accumulation of contaminants in the hardware crevices, supporting clean-room and regulated research standards.

• Process Scale-Up: Provides the necessary hardware support for researchers transitioning to 50 mm ID columns to increase their purification throughput.

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