Falcon Culture Flask 50 ml 0.2 um Vented Cap Total of 100 Flasks

Falcon 353108 25 cm² Cell Culture Flask (TC-Treated, 0.2 µm Vented Cap)

The Falcon 353108 is a high-performance 25 cm² (T-25) cell culture flask designed for the reliable maintenance and expansion of adherent mammalian and insect cell lines. Manufactured from crystal-grade virgin polystyrene, this flask features a proprietary vacuum-gas plasma Tissue Culture (TC) treatment that ensures a uniform, hydrophilic surface for optimal cell attachment and consistent growth kinetics. Equipped with a 0.2 µm hydrophobic vented cap, the flask allows for continuous, sterile gas exchange while providing a secure barrier against particulates and microorganisms. Its compact, canted-neck design provides unobstructed access to the growth surface, making it an essential tool for routine cell maintenance and scale-up workflows.

Technical Performance and Design

• Validated TC-Treated Surface: The proprietary surface modification promotes rapid cell attachment, spreading, and high-density monolayer formation, ensuring phenotypic stability across diverse cell types.

• 0.2 µm Hydrophobic Vented Cap: Features a high-quality microporous membrane that permits uniform O2 and CO2 exchange while preventing internal pressure buildup and protecting the culture from environmental contaminants.

• Canted Neck Geometry: Designed with an angled neck to facilitate easy pipette access and efficient cell harvesting with scrapers, while minimizing "dead zones" at the front of the flask.

• Exceptional Optical Clarity: The high-clarity polystyrene walls ensure a distortion-free view for inverted microscopy, allowing for precise monitoring of cell confluence and morphology.

Product Specifications

• Catalog Number: 353108

• Growth Area: 25 cm²

• Total Flask Volume: 50 ml

• Recommended Working Volume: 5 – 7 ml

• Cap Type: Vented (0.2 µm membrane)

• Sterility: Sterile (Gamma Irradiated); Non-pyrogenic; Non-cytotoxic.

• Packaging: 20 Flasks per Pack / 100 Flasks per Case

Key Benefits & Applications

• Routine Cell Maintenance: The T-25 format is the universal standard for seeding, expanding, and maintaining primary and immortalized cell lines prior to larger-scale experiments.

• Closed-System Sterility: The vented cap allows for consistent incubation conditions without the risk of "cracking" the cap, reducing the likelihood of incubator-borne contamination.

• Safe Transport & Incubation: The stable, stackable footprint prevents sliding and ensures even heat distribution across all flasks within the incubator.

• Traceability & Accuracy: Features molded-in graduations on both sides and a dedicated frosted writing patch to ensure clear sample identification and volume verification.

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