Kontes Kimble Chase Tissue Grinder Set 2 ml with Pour Spout

Kimble Chase 885000-0002 Dounce Tissue Grinder Set – 2 mL, Glass, Dual Pestle

The Kimble Chase 885000-0002 is a precision-engineered 2 mL Dounce tissue grinder designed for the controlled, gentle disruption of animal tissues and cell cultures. Frequently utilized in proteomics and organelle isolation workflows, this set is crafted from high-quality borosilicate glass to ensure chemical inertness and high visibility during the homogenization process. The grinder tube features a wide reservoir and an integrated pour spout, enabling the smooth, splash-free transfer of the resulting homogenate to downstream centrifuge tubes. By utilizing manual vertical strokes, this system allows researchers to achieve high-efficiency cell lysis without the destructive heat and high-shear forces associated with motorized homogenizers.

Technical Performance and Design

• Two-Pestle System (Loose & Tight): This set includes two glass pestles with specific tolerances. The "Loose" pestle (Type A) is used for initial tissue reduction, while the "Tight" pestle (Type B) provides the final, high-clearance stroke to complete cell lysis while preserving organelles.

• Integrated Pour Spout: The mortar is designed with a flared top and a precision pour spout. This feature is critical for the aseptic, quantitative transfer of the sample, ensuring that no precious material is lost or contaminated during decanting.

• Borosilicate Glass Construction: Manufactured from low-expansion borosilicate glass, the set offers excellent resistance to thermal shock and chemical attack. It can be repeatedly autoclaved or dry-heat sterilized for use in RNase-free and sterile environments.

• Precision Clearances: The clearances between the pestles and the tube wall are meticulously controlled during manufacturing to ensure reproducible homogenization results across different laboratory batches and users.

Product Specifications

• Catalog Number: 885000-0002 (Formerly Kontes)

• Product Type: Dounce Tissue Grinder

• Working Capacity: 2 mL

• Material: Type I, Class A Borosilicate Glass

• Included Components: 1 Mortar (Tube), 1 "Loose" Pestle, 1 "Tight" Pestle

• Features: Integrated Pour Spout, Manual Operation

Key Benefits & Applications

• Organelle Isolation: The premier tool for isolating intact nuclei, mitochondria, and enzyme complexes. The gentle "squeezing" action of the Dounce pestle ruptures the plasma membrane without fragmenting the delicate internal structures.

• Suspension Cell Lysis: Highly effective for lysing soft tissues (like liver or brain) and suspension-grown mammalian cells where high-speed mechanical shearing would lead to protein denaturation.

• Repeatable Sample Prep: Because the operation is manual and the glass clearances are fixed, researchers can standardize the number of "strokes" per sample to achieve highly consistent results for Western Blotting or NGS library prep.

All product names, logos, and brands are property of their respective owners.