Macherey-Nagel NucleoSpin PCR Clean-up Pack of 10 Preps

Macherey-Nagel 740609.10 NucleoSpin Gel and PCR Clean-up Kit – 10 Preps

The Macherey-Nagel 740609.10 is a NucleoSpin Gel and PCR Clean-up kit designed for the rapid isolation and purification of DNA fragments from polymerase chain reactions and agarose gels. Utilizing a silica membrane technology within a mini-spin column format, this 2-in-1 kit efficiently removes primers, nucleotides, enzymes, and salts from reaction mixtures, while also allowing for the direct dissolution and extraction of DNA from TAE or TBE agarose slices. This specific catalog number provides 10 complete preparations, making it a standard trial-size format or ideal for low-throughput operations. The proprietary Binding Buffer NTI ensures optimal pH conditions for DNA binding, enabling the recovery of fragments ranging from 50 base pairs up to 20 kilobase pairs.

Technical Performance and Design

• Silica Membrane Technology: The engineered silica matrix strongly binds DNA in the presence of chaotropic salts, allowing enzymes, labels, and mineral oil impurities to be thoroughly washed away using the ethanolic NT3 buffer.

• 2-in-1 Flexibility: Employs a single buffer system (Buffer NTI) capable of dissolving up to 200 mg of agarose gel or neutralizing up to 200 µL of PCR reaction mixtures without requiring separate protocols.

• Low Elution Volume: Designed to release purified DNA with as little as 15 to 30 µL of alkaline elution buffer (Buffer NE), producing highly concentrated samples suitable for direct use in downstream enzymatic reactions.

• pH Optimized Binding: The NTI binding buffer acts as a pH indicator and buffering agent, guaranteeing that the sample matrix remains at the optimal pH (5.0 to 6.0) for maximum DNA adsorption onto the silica membrane.

Product Specifications

• Catalog Number: 740609.10

• Format: Mini Spin Column

• Technology: Silica Membrane

• Sample Capacity: Up to 200 µL (PCR Mixture) or up to 200 mg (Agarose Gel)

• Fragment Size Range: 50 bp to 20 kbp

• Binding Capacity: 25 µg DNA

• Typical Recovery: 70% to 95%

• Elution Volume: 15 µL to 30 µL

• Packaging: 10 Preps (Spin columns and associated buffers included)

Key Benefits & Applications

• Downstream Readiness: Produces high-purity DNA (A260/A280 ratio of 1.8 to 1.9) immediately ready for sensitive applications such as Sanger sequencing, cloning, restriction analysis, and microarray spotting.

• Primer Elimination: Quantitatively removes unincorporated primers (< 50 bp) and primer-dimers from the final eluate, preventing background interference during subsequent sequencing or cloning steps.

• Broad Gel Compatibility: Seamlessly dissolves both standard and low-melting-point agarose gels cast with standard electrophoresis buffers (TAE or TBE).

• Rapid Workflow: Requires only 10 minutes to process a batch of 6 samples, significantly reducing bench time compared to traditional phenol-chloroform extraction or ethanol precipitation.

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