Phenomenex Microplate Polymeric SPE Microelution 96 Well Plate

Phenomenex SPE-BETA-143 Polymeric SPE Microplate – Microelution, 96-Well

The Phenomenex SPE-BETA-143 is a highly specialized Solid Phase Extraction (SPE) microplate engineered for the rapid, high-throughput isolation of target analytes from ultra-low volume biological samples. Configured in a specialized microelution format, each well in the 96-well manifold contains a minimal bed of advanced polymeric sorbent securely held in place by precision bottom frits. Unlike traditional silica-based sorbents, this rugged polymeric backbone resists deconditioning if accidentally dried out during the conditioning or washing steps, entirely eliminating the risk of lost sample recoveries. The microelution design enables analyte elution in volumes as low as 25 µL, completely bypassing the nitrogen dry-down step that frequently causes adsorptive losses of "sticky" analytes to the walls of collection plates. This results in ultra-clean, highly concentrated extracts that are immediately ready for LC-MS/MS analysis.

Technical Performance and Design

• Microelution Efficiency: The ultra-low sorbent mass is explicitly optimized for micro-volume biological samples. It allows for highly concentrated elution volumes (25–50 µL), drastically reducing processing time by completely eliminating the need for solvent evaporation and reconstitution.

• Advanced Polymeric Backbone: The high-surface-area polymeric sorbent prevents the bed from collapsing, ensuring that accidental drying under a vacuum manifold will not compromise sensitive extraction recoveries or waste valuable samples.

• Precision Bottom Frits: Engineered bottom frits strictly contain the tiny polymeric sorbent bed while preventing solvent channeling. This ensures identical flow rates across all 96 wells and completely prevents particulate breakthrough into the final LC-MS/MS extract.

• Direct-to-Vial Workflow: The concentrated extracts can be eluted directly into an analytical collection plate or autosampler vial insert, streamlining the transition from sample preparation directly to the mass spectrometer.

Product Specifications

• Catalog Number: SPE-BETA-143

• Format: 96-Well Microelution Plate

• Phase/Chemistry: Polymeric SPE (Custom / Beta Designation)

• Hardware: Polypropylene with Precision Bottom Frits

• Target Application: Low-Volume Extraction and Method Development

Key Benefits & Applications

• Low-Volume Sample Extraction: The absolute gold standard for pediatric testing, murine (mouse) models, tears, dried blood spots, or any diagnostic assay where the available biological sample volume is strictly limited.

• Eliminates Evaporation Losses: Bypassing the dry-down step is critical for volatile compounds or hydrophobic peptides that frequently stick irreversibly to the plastic walls of collection plates during solvent evaporation.

• Method Development Ready: As a specialized "BETA" phase plate, it offers unique selectivities that assist analytical chemists in overcoming difficult matrix interferences that standard commercial phases cannot resolve.

• High-Throughput Automation: The standard SBS footprint is perfectly optimized for automated liquid handling robots, positive pressure manifolds, and rapid batch processing without manual intervention.

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