Applied Biosystems 384 Well Heated Cover Assy for Thermal Cycler

Applied Biosystems 384-Well Heated Cover Assembly for Thermal Cyclers (A33536)

The Applied Biosystems 384-Well Heated Cover Assembly (A33536) is an essential thermal management component engineered for integration with Applied Biosystems automated thermal cycling and Quantitative Real-Time PCR (qPCR) platform blocks. Designed to maintain continuous, uniform downward thermal pressure across the top surface of 384-well microplates and optical sealing films, this assembly actively prevents sample condensation and vapor phase loss during rapid PCR thermal cycling. Constructed with a high-durability metallic heating element and a precision-contoured contact plate, the A33536 ensures even heat distribution across all 384 reaction wells to eliminate micro-volume evaporation and prevent optical interference during fluorescence signal detection. Engineered as a direct replacement or upgrade module, this assembly maintains exact thermal tolerances and mechanical alignment in high-throughput biopharmaceutical, diagnostic, and academic research laboratories.

Technical Performance & Structural Attributes

• Precision Downward Thermal Pressure: Applies consistent mechanical load and targeted upper thermal energy across sealing films to prevent condensation beneath the well lids.
• 384-Well Uniformity Matrix: Optimized heating element layout delivers homogeneous surface temperatures, protecting reaction volumes across peripheral and central wells alike.
• Rapid Thermal Response: High-performance internal resistive heating array reaches target lid temperatures quickly to match rapid thermal ramp protocols.
• Robust Metallic Contact Plate: Smooth, chemical-resistant contact surface resists wear, corrosion, and optical film residue accumulation during repeated plate loading cycles.
• Plug-and-Play Instrument Integration: Features factory-calibrated quick-connect electrical wiring and mounting interfaces for direct installation onto compatible thermal cycler block housing units.

Product Specifications

• Catalog / Part Number: A33536
• Primary Manufacturer: Applied Biosystems (Thermo Fisher Scientific)
• Equipment Category: Thermal Cycler Sub-Assemblies / Heated Cover Modules
• Block Format Compatibility: 384-Well Thermal Cycler Sample Blocks
• Surface Material: Anodized Aluminum / High-Conductivity Alloy Contact Surface
• Operating Temperature Range: Up to 105 degrees C Heated Lid Temperature Limit
• Function: Condensation Prevention and Reaction Volume Evaporation Control
• Electrical Interface: Integrated Multi-Pin Power & Sensor Harness Connector
• Instrument Compatibility: Designed for Applied Biosystems Automated Thermal Cycler (ATC) and ProFlex / Veriti 384-Well Systems
• Regulatory Status: For Research Use Only (RUO)

Key Benefits & Applications

• High-Throughput qPCR Evaporation Prevention: Maintains low reaction volume stability (5 µL to 20 µL) across 384-well plates during extended amplification protocols.
• Automated Thermal Cycler Maintenance: Restores optimal lid pressure and thermal performance in automated liquid handling and robotic PCR workstations.
• Consistent Optical Signal Readout: Prevents moisture droplet formation on optical seals, eliminating light refraction noise during real-time fluorescence acquisition.

All product names, logos, and brands are property of their respective owners. Applied Biosystems, ProFlex, Veriti, and Thermo Fisher Scientific are registered trademarks of Thermo Fisher Scientific Inc.