Corning Microplate 384 Well Clear Bottom Sealed Pack of 20 Plates

Corning 384-Well Black Clear-Bottom Assay Microplates – Non-Binding Surface (NBS) Treatment, Polystyrene Matrix, Flat Bottom, Non-Sterile (MPN: 3764)

EXPIRATION: 2025-04-17

The Corning Life Sciences 3764 (also cataloged under high-throughput distribution networks as CLS3764 or 3764-CS) is a premium, screening-grade 384-well microplate engineered for top-performance fluorescence and polarization assays where minimizing non-specific protein binding and optical background noise is critical. Injection-molded from ultra-pure virgin polystyrene, this specialized microplate features an opaque black polymer matrix structurally bonded to an optically clear, flat well bottom. This design blocks inter-well light leakage (crosstalk) while allowing for precise bottom-read detection and inverted microscopic evaluation. Pre-treated with Corning's proprietary Non-Binding Surface (NBS) technology, the well walls undergo a non-ionic hydrophilic modification that prevents proteins, nucleic acids, and small hydrophobic molecules from adsorbing to the plastic, securing high signal linearity for ultra-sensitive screening applications.

Technical Performance and Optical Design

• Non-Binding Surface (NBS) Treatment: The internal polystyrene surfaces are modified using a non-ionic hydrophilic chemistry. This prevents target enzymes, proteins, or molecular compounds from sticking to the well walls, optimizing baseline recoveries for low-concentration assays and homogeneous screenings.

• Dual-Matrix Optical Shielding: Fabricated with solid black opaque well sidewalls that absorb excitation light and prevent inter-well signal leakage (crosstalk). The optically transparent flat bottom layer permits maximum light transmission for bottom-reading photomultiplier tubes (PMTs) and plate reader optics.

• High-Density 384-Well Layout: Features a micro-volume 384-well grid spacing that reduces working volume boundaries down to 20–80 µL. This geometry slashes expensive reagent consumption while vastly accelerating processing throughput compared to standard 96-well configurations.

• Automated Platform Geometry: Designed with strict physical tolerances that strictly comply with standard ANSI/SLAS footprint mandates. This guarantees clean compatibility with high-throughput liquid handling arms, automatic microplate stackers, barcoders, and robotic readers.

Product Specifications

• Catalog Number / MPN: 3764 (Cross-listed as CLS3764 / 3764-CS)

• Manufacturer / Brand: Corning Life Sciences

• Format Grid Array: 384-Well Matrix Layout (Standard 16 x 24 row/column structure)

• Volumetric Capacity Parameters: Total well volume capacity of 112 µL / Recommended working assay volume: 20 µL to 80 µL

• Color Silhouette Profile: Opaque Black Frame and Sidewalls with an Optically Clear Flat Bottom

• Surface Treatment Chemistry: Non-Binding Surface (NBS) Modified Polystyrene

• Well Bottom Geometry: Flat Bottom profile for optimal optical read paths

• Sterility Metric: Non-Sterile (Optimized for cell-free biochemical screens, molecular binding assays, and enzymatic loops)

Key Benefits and Applications

• High-Throughput Screen Fluorescence: The premier template for executing sensitive fluorescence intensity, time-resolved fluorescence (TRF), and fluorescence polarization (FP) assays where high signal-to-noise ratios are mandatory.

• Scintillation and Luminescence Profiling: The solid black wall matrix ensures maximum light isolation, eliminating signal bleed-through from high-signal adjacent wells during sensitive luminescent discovery passes.

• Low-Concentration Protein Binding Assays: Excellent choice for running receptor-ligand binding studies, enzyme kinetics, and protein-protein interaction matrices where target compound loss to plastic walls must be suppressed.

• Robust Multi-Layer Storage Stability: Flat structural lips and uniform outer stackers prevent side displacement, allowing robotic hands to store, slide, and read plates without mechanical sticking or jamming faults.

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