Agilent 5188-5321 Glass Tube Inserts 350 ul Total of 800 Tubes

Agilent Technologies 350 microliter Glass Microtube Inserts for 96-Well Microplates – High-Clarity Borosilicate Glass, Flat-Bottom Well Liner, Micro-Sampling (MPN: 5188-5321)

The Agilent Technologies 5188-5321 (also cataloged as 51885321 or Agilent P/N 5188-5321) is a high-purity, inert 350 microliter glass tube insert designed specifically for integration with 96-well microplate platforms (such as Agilent assembly 5065-4402). Precision-molded from high-clarity Type I borosilicate glass, these micro-inserts convert standard polypropylene well plates into inert glass-lined analytical vessels. This prevents active compound adsorption, plasticizer leaching, and organic solvent interaction during high-throughput screening, HPLC, LC-MS, and GC autosampler runs. Engineered with a flat-bottom inner well profile, each 350 microliter tube maximizes volumetric capacity while providing a stable, uniform surface for needle aspiration in automated liquid handling and high-throughput chromatography systems.

Technical Performance and Structural Design

• Inert Type I Borosilicate Glass Construction: Fabricated from low-extractable borosilicate glass to eliminate sample-to-plastic interactions, preventing analyte loss due to non-specific surface adsorption or solvent-induced leaching of plastic additives.

• 96-Well Plate Integration Geometry: Dimensions are engineered specifically to fit inside deep-well microplate cavities, transforming 96-well polypropylene plates into chemically inert glass micro-vial arrays.

• Flat-Bottom Micro-Sampling Reservoir: Features a precision flat bottom that maximizes usable internal working volume up to 350 microliters while allowing autosampler needles to draw low-volume samples cleanly without bottom collision risks.

• High-Throughput Autosampler Compatibility: Optimized for seamless operation inside Agilent 1100, 1200, 1260, and 1290 Infinity Series HPLC/LC-MS autosamplers and fraction collectors configured for well plate sampling.

Product Specifications

• Part Number / MPN: 5188-5321 (Cross-listed as 51885321 / Agilent 5188-5321)

• Manufacturer / Brand: Agilent Technologies

• Component Classification: Glass Microtube Inserts for 96-Well Plates (Not for standard 2 mL autosampler vials)

• Nominal Volumetric Capacity: 350 microliters (0.35 mL)

• Material Composition: Ultra-Clear Type I Borosilicate Glass

• Base / Well Bottom Geometry: Flat Bottom

• System Interoperability: Fits 96-well plate assemblies (such as Agilent 5065-4402) and uses matching cap/septa mats (Agilent 5188-5322)

• Packaging Standard: Bulk pack of 960 (or 1000) individual glass inserts per package

Key Benefits and Applications

• High-Throughput LC-MS & HPLC Bioanalysis: The gold standard choice for bioanalytical CROs and pharmaceutical research labs running aggressive organic solvents (such as DCM, chloroform, or DMSO) that would degrade standard plastic microplates.

• Trace Analyte & Small Molecule Recovery: Prevents hydrophobic drug candidates, steroids, and active pharmaceutical ingredients (APIs) from sticking to plastic well walls, ensuring high quantitative recovery.

• Cost-Effective Plate Re-Capping: Allows laboratory technicians to replace individual glass tube inserts in reusable 96-well microplate frames without replacing the entire plate assembly.

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