Corning 3471 Costar Microplate 6 Well Ultra-Low Attachment Surface 16.8 ml Polystyrene Case of 24 Plates

Corning® Costar® 6-Well Clear Flat-Bottom Ultra-Low Attachment (ULA) Microplates with Lids (3471) – Case of 24

The Corning® Costar® 3471 is a specialized, research-grade multi-well plate engineered for the generation and expansion of 3D multicellular tumor spheroids (MCTS), embryoid bodies, organoids, and primary stem cell aggregates. Precision-molded from high-purity virgin polystyrene, each plate features a 6-well configuration with an optically clear, flat-bottom base. The interior growth surface of each well is permanently modified with a covalently bound, hydrophilic, neutrally charged Ultra-Low Attachment (ULA) hydrogel layer. This hydrogel coating minimizes non-specific protein adsorption, completely preventing anchorage-dependent cell lines from grounding, flattening, or initiating surface-mediated differentiation. Supplied as a commercial case of 24 individually wrapped plates with matching lids, this model delivers absolute aseptic performance for advanced oncology, immunology, and stem cell bioprocessing cleanrooms.

Technical Performance & Surface Chemistry

• Covalently Bound Hydrogel Layer: Features a stable, ultra-thin hydrogel matrix cross-linked to the polystyrene well bottom. This non-ionic, neutral barrier suppresses the adsorption of key serum attachment proteins (such as fibronectin and vitronectin), effectively neutralizing cell-to-plastic adhesion forces.
• 3D Spheroid & Aggregate Optimization: By denying a physical grounding plane, cells are maintained in a continuous suspension state. This forces natural cell-to-cell signaling loops, promoting the spontaneous self-assembly of uniform 3D spheroids and suspension clusters.
• Inverted Optical Clarity Profile: Precision injection-molded flat well bases provide a uniform, thin optical window with low background autofluorescence. This layout ensures crisp, distortion-free focal planes during high-resolution phase-contrast or inverted fluorescence microscopy tracking.
• Condensation-Control Non-Reversible Lid: The included clear polystyrene lid incorporates dedicated condensation rings matching the 6-well matrix. This design ensures safe gas exchange while minimizing localized evaporation rates and preventing cross-contamination across adjacent wells.

Product Specifications

• Catalog Number / MPN: 3471
• Primary Manufacturer: Corning Life Sciences (Costar® Product Family)
• Plate Format Configuration: 6-Well Multi-Well Matrix (2 x 3 Array)
• Well Bottom Profile: Flat-Bottom Geometry (Optimized for microscopy and automated high-resolution scanning)
• Surface Chemistry: Ultra-Low Attachment (ULA) Hydrogel Coating (Neutrally charged, non-ionic surface profile)
• Material Composition: Optical-grade, USP Class VI virgin polystyrene (Latex-free / Silicone-free)
• Total Well Volume Capacity: Nominal 16.8 mL per well
• Recommended Working Volume: 1.9 mL to 2.9 mL per well
• Cell Growth Base Area: Nominal 9.5 cm² per well
• Sterility & Purity Status: Gamma-irradiation sterilized (SAL $10^{-6}$ baseline); Certified Non-Pyrogenic and Non-Cytotoxic
• Packaging Configuration: Case of 24 Plates with Lids (Each plate individually wrapped in an easy-to-peel medical-grade aseptic pouch)

Key Benefits & Applications

• Tumor Spheroid and Organoid Cultivation: The industry benchmark platform for generating consistent 3D tumor models directly from primary tumor tissues or established cancer cell lines for high-accuracy drug screening.
• Stem Cell Maintenance: Prevents early, surface-mediated differentiation of human pluripotent stem cells (hPSCs/iPSCs), maintaining stemness markers during early cluster expansion or multi-lineage differentiation staging.
• Automation and Robotic Stacking: Built to meet universal ANSI/SLAS footprint standard specifications, ensuring smooth, jam-free integration with automated microplate washers, liquid handlers, and robotic incubator tracks.
• **Lot-Specific Compliance Tracking:** Every sterile blister pouch has the master manufacturing lot code and expiration metric printed directly on the exterior liner to facilitate cleanroom auditing and institutional inventory compliance.

Expiration Date 2025-11-02

Corning® and Costar® are registered commercial trademarks of Corning, Inc. Catalog number 3471 specifies the flat-bottom, Ultra-Low Attachment 6-well setup provided with a lid. For routine attachment-dependent cell cultures or standard monolayer expansion workflows, companion tissue culture-treated (TC) multi-well configurations (such as Costar SKU 3516) must be selected instead.