Corning Cell Culture Plate 12 Well Clear Sterile TC-Treated Case of 100 Plates

Corning Costar 12-Well Cell Culture Plates – Clear Flat Bottom, Optically Transparent Virgin Polystyrene, Uniform Tissue Culture-Treated, with Vented Lid, Sterile (MPN: 3512)

The Corning Life Sciences 3512 (also cataloged across international biomedical networks as CLS3512 or 3512-CS) is a premium, research-grade multiwell cell culture plate engineered to drive robust anchorage, uniform monolayer development, and high-viability expansion of adherent mammalian cells. Injection-molded from ultra-pure, crystal-clear virgin polystyrene, this 12-well plate features an standard 3 x 4 grid layout with flat-bottom wells designed for optical consistency. Each well undergoes a uniform vacuum-gas plasma Tissue Culture (TC) surface treatment, infusing hydrophilic, negatively charged oxygen groups into the plastic matrix to optimize cell attachment and spreading. Arriving gamma-sterilized and equipped with a specialized condensation-ringed lid, this plate incorporates an alphanumeric indexing grid and individual raised well rims to minimize cross-well contamination and edge-effect evaporation during long incubations.

Technical Performance and Structural Design

• Hydrophilic Vacuum-Plasma TC Treatment: The cell growth surfaces are permanently modified via a validated gas-plasma treatment. This uniform chemistry drives highly consistent cell adhesion, uniform monolayer spreading, and excellent proliferation rates across anchorage-dependent primary lines.

• Advanced Evaporation and Crosstalk Defense: Engineered with individual condensation rings molded into the clear lid that align precisely over each well headspace. These rings intercept evaporating moisture loops, while raised well rims isolate each chamber to prevent cross-contamination.

• Crystal-Clear Optical Path Length: Fabricated from high-transmission, optical-grade polystyrene. The perfectly flat bottom surfaces ensure distortion-free visual inspection, high-magnification inverted microscopy tracking, and clean vertical spectrophotometric reader scans.

• Validated Medical-Grade Purity: Sterilized using validated gamma irradiation protocols to secure an absolute aseptic state. Every factory lot is certified non-pyrogenic (endotoxin levels less than 0.1 EU/mL) and non-cytotoxic to safeguard sensitive cellular lines.

Product Specifications

• Catalog Number / MPN: 3512 (Cross-listed as CLS3512 / 3512-CS)

• Manufacturer / Brand: Corning Life Sciences / Costar®

• Plate Format Geometry: 12-Well Grid Layout (Standard 3 x 4 matrix meeting ANSI/SLAS dimensional footprints)

• Well Dimensions Detail: Well Diameter: 22.1 mm / Total growth area: 3.8 square centimeters ($3.8\ \text{cm}^2$) per well

• Volumetric Capacity Parameters: Total well volume capacity of 6.0 mL / Recommended operational working volume: 1.9 mL to 2.9 mL

• Material Composition: Ultra-Pure Crystal Clear Virgin Polystyrene (PS)

• Surface Chemistry: Hydrophilic Tissue Culture-Treated (TC-Treated) for adherent cell growth

• Included Lid Closure Profile: Clear Polystyrene Low-Evaporation Vented Lid featuring orientation corners and an anti-slip stacking lip

Key Benefits and Applications

• Adherent Cell Scale-Up and Seeding: The ideal intermediate matrix scale for expanding primary cell lines, stem cells, and established adherent cultures before harvesting for macro-volume bioreactors.

• Transfection and Transwell Permeability Assays: The 22.1 mm well diameter provides perfect geometric compatibility for standard 12 mm Transwell membrane inserts, supporting complex co-culture and drug transport lines.

• Automated Liquid Handling Compatibility: Extremely rigid external boundaries conform to automated microplate stackers, barcoders, liquid handlers, and robotic plate readers without mechanical nesting errors.

• Aseptic Single-Direction Alignment: The notched corner design of the lid ensures it can only be placed on the plate in one direction, preventing cross-contamination errors and row-tracking mistakes.

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