Cytiva Prepacked Column HiTrap Capto Q 5 ml Lot of 3 Sealed Columns

The Cytiva HiTrap Capto Q (11-0013-03) is a 5 mL prepacked column designed for preparative Ion Exchange Chromatography (IEX). It is packed with Capto Q resin, a strong quaternary ammonium (Q) anion exchanger that is a mainstay in the purification of proteins, nucleic acids, and other biomolecules. The "Capto" platform represents a high-productivity resin designed for large-scale bioprocessing; it utilizes a high-flow agarose base matrix with a rigid structure that allows for significantly higher flow rates and lower backpressures than traditional Sepharose media. This makes it ideal for capturing targets from large volumes of crude feedstock where speed is essential to prevent sample degradation.

The 5 mL volume of the HiTrap column is an industry standard for "method scouting" and small-scale purification. These columns are designed for ease of use, featuring a simple polypropylene housing that can be operated via a syringe, a peristaltic pump, or integrated directly into automated chromatography systems like the ÄKTA platform. The resin itself is "strong," meaning it remains fully charged and maintains its high binding capacity across a broad pH range (typically pH 2–12), allowing for greater flexibility during process development. Because this is a sealed lot of 3 columns, it provides a convenient, ready-to-use supply for researchers who need to ensure reproducibility between different purification runs. The columns are typically stored in 20% ethanol to maintain the integrity of the resin and the sterility of the fluid path until they are primed for use.