Repligen MidiKros Hollow Fiber Filter 20 cm Length 0.5 um MPES 0.5 mm

Repligen D02-E500-05-N MidiKros Tangential Flow Filtration (TFF) Hollow Fiber Filter Module (20 cm, 0.5 µm MPES, 0.5 mm ID). A single-use / reusable hollow fiber tangential flow filtration cartridge engineered for gentle, low-shear microfiltration, mammalian and microbial cell harvesting, and particulate clarification in laboratory- and pilot-scale bioprocessing workflows.

• MPN / Catalog Model: D02-E500-05-N
• Manufacturer & Line: Repligen (formerly Spectrum Labs) / MidiKros Hollow Fiber TFF Filter Module Series
• Membrane Chemistry & Material: Modified Polyethersulfone (mPES / MPES); exhibits extremely low non-specific protein binding, minimal fouling, and broad biocompatibility for high product yield
• Pore Size Rating: 0.5 µm microfiltration membrane rating (ideal for whole-cell retention, fine particle separation, and crude lysate clarification)
• Fiber Geometry & Dimensions:
  • Effective Length: 20 cm path length
  • Fiber Inner Diameter (ID): 0.5 mm (500 µm lumen diameter), providing an optimal balance between hydrodynamic shear control and flux in low-to-medium viscosity feeds
• Housing & Potting Construction: Translucent polysulfone (PS) outer shell with medical-grade polyurethane potting resin, delivering high chemical stability, mechanical integrity, and visibility of fluid paths during processing
• Inlet / Outlet & Permeate Ports: Standard sanitary sanitary fittings or Luer-lock fluid connectors (standard MidiKros porting) designed for leak-free, reproducible hookup into automated and manual peristaltic TFF skids (e.g., KrosFlo systems)
• Processing Volume Range: Optimized for laboratory, benchtop, and small-batch pilot processing volumes typically ranging from 100 mL to 3 L
• Sterilization & Storage Conditions: Non-pyrogenic; supplied ready-to-use with standard non-sterile preservation ("-N" non-irradiated designation) and compatible with standard sanitize-in-place (SIP) protocols using dilute sodium hydroxide (NaOH) or sodium hypochlorite solutions
• Primary Application: Tangential flow microfiltration (TFF/crossflow), mammalian cell harvesting, bacterial and yeast clarification, viral vector (AAV, lentivirus) recovery, lysate de-bulking, perfusion cell retention, and micro-carrier separation