Tecan Microplate Reader 415 nm Filter for GENios Series

The 415 nm filter for the Tecan GENios series is a specialized optical component used primarily for absorbance measurements and specific fluorescence excitation protocols. In the GENios architecture, these filters are mounted into specialized excitation or emission filter slides.

Core Applications

The 415 nm wavelength is essential for several high-value laboratory protocols:

• ELISA Development: Commonly used as a secondary wavelength for detecting ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] substrates, which have an absorbance peak near 415 nm.
• Heme & Hemoglobin Quantification: This wavelength sits near the Soret band, making it highly effective for measuring hemoglobin concentration or monitoring heme-protein interactions in biochemical studies.
• Beta-Galactosidase Assays: Used in reporter gene studies (such as LacZ) when utilizing specific colorimetric substrates that yield a yellow-green product.
• Fluorescence Excitation: Acts as an excitation source for dyes like Lucifer Yellow or certain quantum dots that require violet-blue light.

Used; excellent condition.