Tecan Microplate Reader 550 nm Filter for GENios Series

The 550 nm filter for the Tecan GENios series is a standard optical component typically used for absorbance measurements and fluorescence emission. In the GENios architecture, these filters are mounted into specialized excitation or emission filter slides.

Core Applications

The 550 nm wavelength is vital for several common laboratory readouts:

• Absorbance (BCA Assay): Used for protein quantification when using the BCA (Bicinchoninic Acid) assay, where the green-to-purple color change of the substrate is measured (peak absorbance is near 562 nm, but 550 nm is a frequently used standard bandwidth).

• Fluorescence Emission (Cy3 & TRITC): Serves as a primary emission filter for orange-red fluorophores like Cy3, TRITC, and TAMRA. It is typically paired with a 530 nm or 535 nm excitation filter.

• Colorimetric Viability (MTT): While 570 nm is the peak for MTT formazan, 550 nm is often used as a reference or primary wavelength for measuring cell proliferation and metabolic activity in 96-well formats.

• Phenol Red Monitoring: Used to measure the pH status of cell culture media based on the absorbance of phenol red.

Used; excellent condition.