Thermo Scientific Cell Culture Microplate 96 Well V Bottom Case of 50 Plates

Thermo Scientific™ Nunc™ 96-Well Clear V-Bottom Tissue Culture-Treated Microplates with Lids (249662) – Case of 50

The Thermo Scientific™ Nunc™ 249662 Microplate is a specialized, research-grade multi-well consumable designed for high-throughput sample concentration, cellular pelleting, sequencing preparation, and automated liquid handling workflows. Precision-molded from highly transparent virgin polystyrene, this plate incorporates a 96-well matrix characterized by an engineered conical V-bottom well profile. This V-shaped baseline optimizes fluid recovery by funneling liquid toward the absolute lower vertex of each well, significantly reducing residual dead volumes. The interior base of each well undergoes a uniform vacuum-gas plasma tissue culture (TC) surface treatment, promoting consistent cell attachment, spreading, and monolayer formation. Supplied as a commercial case of 50 individually sealed plates with matching lids, this sterile configuration delivers robust aseptic performance for clinical, industrial bioprocessing, and academic screening lines.

Technical Performance & Design Architecture

• Fluid-Optimized V-Bottom Geometry: The steep conical incline of each well base concentrates precipitates, cell pellets, and sparse reagents into a tight micro-localized zone at the bottom center. This configuration maximizes liquid extraction margins during multi-channel aspirating loops.
• Vacuum-Plasma Surface Activation: The cell-contact matrix is modified with hydrophilic oxygen groups to facilitate uniform anchorage-dependent cell adhesion. This minimizes well-to-well variability and edge effects during monolayer expansion or sequential washing assays.
• Patented Airvent Moisture Protection: The included clear polystyrene lid uses a non-reversible structural layout paired with custom condensation rings. This physical alignment allows stable gas exchange during multi-day incubation cycles while controlling well-to-well vapor cross-contamination and micro-evaporation.
• Individually Wrapped Sterile Pouches: Every plate and lid pair is gamma-irradiated and sealed in an individual polymer peel-pack. This ensures a pristine, nuclease-free barrier, allowing technicians to introduce single plates into cleanroom biosafety cabinets without exposing raw inventory stocks to ambient pollutants.

Product Specifications

• Catalog Number / MPN: 249662
• Primary Manufacturer: Thermo Fisher Scientific (Nunc™ Product Portfolio)
• Plate Format Configuration: 96-Well Matrix (8 x 12 Array)
• Well Bottom Profile: Conical / V-Bottom Base (Optimized for sample retrieval, pelleting, and separation)
• Surface Chemistry: Standard Tissue Culture (TC) Treated (Hydrophilic matrix)
• Material Composition: Premium USP Class VI Virgin Polystyrene (PS) | Certified Latex- and Silicone-Free
• Total Volumetric Well Capacity: Nominal 300 µL per well
• Recommended Working Volume: 50 µL to 250 µL per well
• Cell Growth Base Area: Nominal 0.25 cm² per well
• Sterility & Purity Standards: Gamma-irradiation sterilized; Certified Non-Pyrogenic and Non-Cytotoxic
• Packaging Configuration: Case of 50 Plates with Lids (Individually bagged units packed securely within protective warehouse distribution cartons)

Key Benefits & Applications

• High-Recovery Cold Centrifugation & Pelleting: Engineered to withstand routine plate-rotor g-forces, making it an excellent vehicle for collecting non-adherent cell clusters, blood components, or nucleic acid precipitates.
• Automated Liquid Handling Compatibility: Modeled to strictly adhere to standard ANSI/SLAS footprint profiles, ensuring precise physical integration with automated pipetting decks, microplate washers, and optical reader carousels.
• Sample Staging and Serial Dilution Runs: Provides reliable precision when performing automated compound screening, immunological dilutions, or aliquoting genomic master mixes where expensive reagents must be fully reclaimed.
• Alphanumeric Identification Mapping: Features high-contrast, molded-in alphanumeric tracking labels along rows A–H and columns 1–12, facilitating quick verification of target wells during dense manual or automated loading protocols.

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