{"product_id":"thermo-scientific-249662-cell-culture-microplate-96-well-v-bottom","title":"Thermo Scientific Cell Culture Microplate 96 Well V Bottom Case of 50 Plates","description":"\u003ch3\u003eThermo Scientific™ Nunc™ 96-Well Clear V-Bottom Tissue Culture-Treated Microplates with Lids (249662) – Case of 50\u003c\/h3\u003e\n\u003cp\u003eThe Thermo Scientific™ Nunc™ 249662 Microplate is a specialized, research-grade multi-well consumable designed for high-throughput sample concentration, cellular pelleting, sequencing preparation, and automated liquid handling workflows. Precision-molded from highly transparent virgin polystyrene, this plate incorporates a 96-well matrix characterized by an engineered conical V-bottom well profile. This V-shaped baseline optimizes fluid recovery by funneling liquid toward the absolute lower vertex of each well, significantly reducing residual dead volumes. The interior base of each well undergoes a uniform vacuum-gas plasma tissue culture (TC) surface treatment, promoting consistent cell attachment, spreading, and monolayer formation. Supplied as a commercial case of 50 individually sealed plates with matching lids, this sterile configuration delivers robust aseptic performance for clinical, industrial bioprocessing, and academic screening lines.\u003c\/p\u003e\n\u003ch3\u003eTechnical Performance \u0026amp; Design Architecture\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eFluid-Optimized V-Bottom Geometry: The steep conical incline of each well base concentrates precipitates, cell pellets, and sparse reagents into a tight micro-localized zone at the bottom center. This configuration maximizes liquid extraction margins during multi-channel aspirating loops.\u003c\/li\u003e\n\u003cli\u003eVacuum-Plasma Surface Activation: The cell-contact matrix is modified with hydrophilic oxygen groups to facilitate uniform anchorage-dependent cell adhesion. This minimizes well-to-well variability and edge effects during monolayer expansion or sequential washing assays.\u003c\/li\u003e\n\u003cli\u003ePatented Airvent Moisture Protection: The included clear polystyrene lid uses a non-reversible structural layout paired with custom condensation rings. This physical alignment allows stable gas exchange during multi-day incubation cycles while controlling well-to-well vapor cross-contamination and micro-evaporation.\u003c\/li\u003e\n\u003cli\u003eIndividually Wrapped Sterile Pouches: Every plate and lid pair is gamma-irradiated and sealed in an individual polymer peel-pack. This ensures a pristine, nuclease-free barrier, allowing technicians to introduce single plates into cleanroom biosafety cabinets without exposing raw inventory stocks to ambient pollutants.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProduct Specifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eCatalog Number \/ MPN: 249662\u003c\/li\u003e\n\u003cli\u003ePrimary Manufacturer: Thermo Fisher Scientific (Nunc™ Product Portfolio)\u003c\/li\u003e\n\u003cli\u003ePlate Format Configuration: 96-Well Matrix (8 x 12 Array)\u003c\/li\u003e\n\u003cli\u003eWell Bottom Profile: Conical \/ V-Bottom Base (Optimized for sample retrieval, pelleting, and separation)\u003c\/li\u003e\n\u003cli\u003eSurface Chemistry: Standard Tissue Culture (TC) Treated (Hydrophilic matrix)\u003c\/li\u003e\n\u003cli\u003eMaterial Composition: Premium USP Class VI Virgin Polystyrene (PS) | Certified Latex- and Silicone-Free\u003c\/li\u003e\n\u003cli\u003eTotal Volumetric Well Capacity: Nominal 300 µL per well\u003c\/li\u003e\n\u003cli\u003eRecommended Working Volume: 50 µL to 250 µL per well\u003c\/li\u003e\n\u003cli\u003eCell Growth Base Area: Nominal 0.25 cm² per well\u003c\/li\u003e\n\u003cli\u003eSterility \u0026amp; Purity Standards: Gamma-irradiation sterilized; Certified Non-Pyrogenic and Non-Cytotoxic\u003c\/li\u003e\n\u003cli\u003ePackaging Configuration: Case of 50 Plates with Lids (Individually bagged units packed securely within protective warehouse distribution cartons)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Benefits \u0026amp; Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eHigh-Recovery Cold Centrifugation \u0026amp; Pelleting: Engineered to withstand routine plate-rotor g-forces, making it an excellent vehicle for collecting non-adherent cell clusters, blood components, or nucleic acid precipitates.\u003c\/li\u003e\n\u003cli\u003eAutomated Liquid Handling Compatibility: Modeled to strictly adhere to standard ANSI\/SLAS footprint profiles, ensuring precise physical integration with automated pipetting decks, microplate washers, and optical reader carousels.\u003c\/li\u003e\n\u003cli\u003eSample Staging and Serial Dilution Runs: Provides reliable precision when performing automated compound screening, immunological dilutions, or aliquoting genomic master mixes where expensive reagents must be fully reclaimed.\u003c\/li\u003e\n\u003cli\u003eAlphanumeric Identification Mapping: Features high-contrast, molded-in alphanumeric tracking labels along rows A–H and columns 1–12, facilitating quick verification of target wells during dense manual or automated loading protocols.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003cp\u003e\u003csmall\u003eAll product names, logos, and brands are property of their respective owners.\u003c\/small\u003e\u003c\/p\u003e","brand":"Thermo Scientific","offers":[{"title":"Default Title","offer_id":49261422149884,"sku":"10886","price":142.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0627\/8463\/5132\/files\/thermo-scientific-cell-culture-microplate-96-well-v-bottom-case-of-50-plates-petri-dishesculture-plates-thermo-scientific-592454.jpg?v=1783525216","url":"https:\/\/lifesciencestrading.com\/es\/products\/thermo-scientific-249662-cell-culture-microplate-96-well-v-bottom","provider":"Life Sciences Trading","version":"1.0","type":"link"}