Thermo Scientific Microplate 96 Well 450 ul Polypropylene Case of 120 Plates

Thermo Scientific Nunc MicroWell 96-Well Storage Plate – 450 µL, Natural Polypropylene, Conical V-Bottom, Non-Sterile (MPN: 249944)

The Thermo Scientific Nunc 249944 is a commercial-grade, standard-height 96-well microplate precision-engineered for high-recovery sample storage, compound library management, chemical liquid-phase assays, and high-throughput screening (HTS). Molded from premium, ultra-clear natural virgin polypropylene (PP), this low-binding assay plate features an 8 × 12 well configuration with a total maximum well capacity of 450 µL (with a nominal safe working volume of 400 µL). The specialized conical V-bottom geometry works in tandem with a unique high-flange, shared-wall structural layout to improve mixing fluid dynamics and achieve complete liquid recovery, making it highly effective for low-volume sample automation lines where sample carryover must be minimized.

Technical Performance and Design

• Conical V-Bottom Apex Geometry: The steep, uniform V-shaped well base concentrates fluids at the absolute center of the bottom. This architecture minimizes capillary hold-up and residual dead volume, delivering exceptional sample recovery parameters during automated aspiration passes.

• Space-Saving Shared-Wall Technology: Engineered with a specialized thin shared-wall configuration that broadens the well orifice diameter while maintaining standard microplate pitches. This increases the total well volume capacity to 450 µL while optimizing lateral mixing efficiency.

• Low-Binding Virgin Polypropylene: Formulated from high-purity, naturally hydrophobic polypropylene resin. This matrix prevents non-specific binding of fragile proteins, peptides, or genomic DNA templates to the plastic surface, securing accurate assay concentrations.

• High Flange Automation Footprint: Designed with a rigid high-flange exterior edge. This reinforcement resists mechanical bowing or warp under high centrifugation lines, ensures steady stacker track feed-through, and provides a stable surface for plate gripper arms.

Product Specifications

• Catalog Number / MPN: 249944

• Manufacturer / Brand: Thermo Scientific / Nunc™ MicroWell™

• Format Layout: Standard 96-Well Matrix (8 × 12 Grid Array)

• Total Volumetric Capacity: 450 µL Per Well (Working Volume: 10 µL to 400 µL)

• Well Bottom Profile: Conical V-Bottom / Round Well Openings

• Material Composition: Translucent Natural Virgin Polypropylene (PP)

• Sterility Status: Non-Sterile, Non-Treated Surface (Fully Autoclavable up to 121°C)

• Thermal Endurance Horizon: Stable from ultra-low deep freeze at -80°C up to 121°C

• Purity Metrics: Certified free of detectable RNase and DNase contaminants

Key Benefits & Applications

• Genomics & PCR Sample Processing: The clean, nuclease-free polypropylene build serves as an excellent mother-plate template for setting up qPCR master mixes, multi-channel template distributions, and NGS sequencing libraries.

• Biobanking & Library Storage: Highly valued for archiving compound libraries, active small-molecule drugs, or analytical extracts due to its outstanding resistance to aggressive solvents like Dimethyl Sulfoxide (DMSO) and alcohols.

• Filtrate Plate Receptacle: Perfectly sized and contoured to function as a collection or receiver plate positioned beneath multi-well vacuum filtration manifolds or standard 96-well Nunc Filter Plates.

• Hermetic Evaporation Barriers: Features uniform, level well rims that distribute compression evenly to generate an evaporation-proof physical seal when paired with ALPS thermal heat-sealing foils or adhesive tape sheets.

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