BIA Separations CIMmultus QA Monolithic Column 1 ml 6 um Pore Size

BIA Separations 311.5113-6 CIMmultus QA Monolithic Column – 6 µm Pore, Strong Anion Exchange

The BIA Separations 311.5113-6 is a high-productivity monolithic column engineered for the preparative and analytical purification of negatively charged biomolecules. Utilizing QA (Quaternary Amine) strong anion exchange chemistry, this column provides a permanent positive charge that remains stable across a wide pH range (2–13). The CIM (Convective Interaction Media) monolithic architecture features a 6 µm pore size, which provides a high-surface-area, flow-through environment that is ideal for very large bioparticles. Because mass transfer is convective rather than diffusive, this column maintains exceptionally high resolution and binding capacity even at ultra-high flow rates. The 311.5113-6 is a gold-standard tool for the downstream processing of plasmid DNA (pDNA), large viral vectors, and non-enveloped viruses.

Technical Performance and Design

• Convective Flow Dynamics: The interconnected monolithic channels eliminate stagnant zones and diffusion limitations, allowing for incredibly fast processing times without compromising the separation profile.

• Strong Anion Exchange (QA) Chemistry: Features a quaternary ammonium functional group that ensures high-capacity binding of negatively charged species, such as nucleic acids and most viral capsids at physiological pH.

• Optimized 6 µm Macro-Porous Structure: Specifically designed to accommodate large biological entities, reducing backpressure and minimizing the risk of "plugging" when processing complex biological feedstocks.

• Shear-Stress Reduction: The smooth, open-channel flow path provides a gentle environment for shear-sensitive viruses and large DNA molecules, maximizing the recovery of active and intact biological products.

Product Specifications

• Catalog Number: 311.5113-6

• Chemistry: QA (Quaternary Amine / Strong Anion Exchange)

• Pore Size: 6 µm

• Ports: 10-32 UNF Coned Port

• Tubing Compatibility: 1/16 in. OD Tubing Connection

• Housing: High-performance plastic housing (Bio-compatible)

• Operating pH Range: 2 – 13

Key Benefits & Applications

• Plasmid DNA (pDNA) Purification: The high capacity for large molecules makes this column exceptionally efficient for the "capture" step in pDNA manufacturing for gene therapy.

• Viral Vector Downstream Processing: Ideal for the purification of Adenovirus, AAV, and Lentivirus, where the QA chemistry helps in the separation of full vs. empty capsids.

• Scalable Chromatography: Part of the CIMmultus family, ensuring that protocols developed on this lab-scale column can be directly transferred to large-scale production monoliths with predictable results.

• Ease of Integration: Features standard 10-32 UNF ports for immediate "plug-and-play" compatibility with AKTA, Bio-Rad, and other common FPLC/HPLC systems.

All product names, logos, and brands are property of their respective owners.