Biotage Isolute C8 2 ml 100 mg Each Tube Pack of 100 Columns

Biotage 290-0100-T ISOLUTE C8 SPE Column – 2 mL, 100 mg

The Biotage 290-0100-T is an ISOLUTE C8 Solid Phase Extraction (SPE) column configured in a 2 mL tube format, specifically designed for the modular ISOLUTE Array plate system. Each tube contains 100 mg of a silica-based sorbent covalently bonded with octyl (C8) hydrocarbon chains. Unlike exhaustively end-capped sorbents, this standard C8 phase is purposely left non-endcapped. This design provides primary non-polar (hydrophobic) retention via the C8 chains, alongside secondary polar and weak cation-exchange interactions from the active surface silanols. Because the C8 alkyl chain is shorter than a traditional C18 phase, it offers a less retentive surface, making it ideal for the extraction of highly lipophilic analytes that would bind too tightly to a C18 column.

Technical Performance and Design

• Dual Retention Mechanism: The non-endcapped surface combines primary hydrophobic retention with secondary weak cation-exchange interactions, providing unique selectivity for basic drugs and polar metabolites in complex aqueous matrices.

• Moderate Hydrophobicity: The shorter octyl (C8) chains require a lower percentage of organic solvent to elute target analytes compared to C18 phases, preventing the irreversible binding of highly non-polar compounds.

• Silica Substrate: The standardized particle size and pore geometry ensure consistent liquid flow rates when processed on standard vacuum manifolds or positive pressure systems.

• 2 mL Array Format: The 2 mL reservoir accommodates small aqueous sample volumes while utilizing a minimal 100 mg sorbent bed. The modular Array design allows researchers to populate only the required number of wells in a 96-well base plate, reducing consumable waste.

Product Specifications

• Catalog Number: 290-0100-T

• Product Line: ISOLUTE C8 (Non-Endcapped)

• Format: 2 mL SPE Tube (ISOLUTE Array Format)

• Sorbent Mass: 100 mg per tube

• Retention Mechanism: Mixed-Mode (Non-polar and Weak Cation Exchange)

• Target Analytes: Highly lipophilic compounds and basic drugs

Key Benefits & Applications

• Bioanalytical Testing: Excellent for the extraction of basic pharmaceutical drugs and their polar metabolites from biological fluids such as plasma and urine.

• Enhanced Elution Efficiency: Perfect for applications where analytes are so highly retained on C18 columns that they cannot be efficiently eluted, ensuring high recovery rates for lipophilic targets.

• Method Development: The modular Array tubes can be mixed and matched with different sorbent chemistries on a single 96-well base plate, streamlining phase-screening protocols.

• Solvent Conservation: The compact 100 mg bed mass combined with the less retentive C8 phase significantly reduces solvent consumption during the washing and elution phases, shortening subsequent evaporation and dry-down times.

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