Biotage SPE Microplate Isolute HAX 96 Well 25 mg Sorbent Plate

Biotage 903-0025-P01 ISOLUTE HAX 96-Well SPE Plate – 25 mg/well

The 903-0025-P01 is an ISOLUTE HAX Solid Phase Extraction (SPE) 96-well plate engineered by Biotage for the extraction of acidic drugs and metabolites from biological fluids. Each well contains 25 mg of a mixed-mode sorbent that combines strong anion exchange and non-polar (hydrophobic) retention chemistries. This dual-retention mechanism allows acidic analytes to bind firmly to the sorbent, enabling rigorous wash steps that selectively strip away endogenous matrix interferences like proteins and neutral lipids. By utilizing this mixed-mode approach, researchers can generate ultra-clean sample extracts suitable for trace-level LC-MS/MS quantification when processing complex matrices like human plasma or urine.

Technical Performance and Design

• Dual Retention Mechanism: Combines hydrophobic and strong anion exchange interactions, allowing analytes with both non-polar and acidic characteristics to be retained while interferences are selectively washed away.

• Robust Interference Elution: The secondary anion exchange mechanism permits the use of aggressive wash regimes with polar and non-polar solvents without causing premature analyte breakthrough.

• Fixed 96-Well Architecture: The standardized plate footprint interfaces with automated liquid handlers, vacuum manifolds, and positive pressure systems (such as the Biotage PRESSURE+ 96) for high-throughput parallel processing.

• Matrix-Independent Retention: The initial non-polar retention of analytes is unaffected by the high or variable ionic strength of the biological sample matrix, ensuring reproducible extraction recoveries.

Product Specifications

• Catalog Number: 903-0025-P01

• Product Line: ISOLUTE HAX

• Format: 96-Well Fixed Plate

• Sorbent Mass: 25 mg per well

• Retention Mechanism: Mixed-Mode (Non-Polar & Strong Anion Exchange)

• Target Analytes: Acidic Compounds

Key Benefits & Applications

• Bioanalytical Testing: Used for extracting acidic pharmaceutical drugs and biomarkers from complex biological fluids prior to liquid chromatography-tandem mass spectrometry (LC-MS/MS).

• Enhanced Extract Purity: Reduces ion suppression and mass spectrometer source fouling by actively excluding neutral and basic endogenous interferences during the wash phases.

• Forensic Toxicology: Isolates targeted acidic compounds from contaminated urine samples, utilizing acidic loading conditions to prevent the retention of endogenous small organic acids.

• Workflow Automation: The rigid well design maintains uniform flow rates across all 96 positions, preventing channeling or vacuum leaks during automated extraction protocols.

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