Biotage Microplate Isolute SAX 96 Well 25 mg Each Well Sealed Plate

Biotage 500-0025-P01 ISOLUTE SAX 96-Well SPE Plate – 25 mg/well

The Biotage 500-0025-P01 is an ISOLUTE SAX Solid Phase Extraction (SPE) 96-well plate engineered for the extraction of acidic compounds from aqueous or partially aqueous samples. Each well contains 25 mg of a silica-based sorbent modified with a quaternary amine functional group. Because this strong anion exchange (SAX) sorbent maintains a permanent positive charge across the entire pH range, it binds strongly to acidic analytes, including strong acids, weak acids, and polyanions. This retention mechanism allows researchers to apply rigorous wash steps to eliminate matrix interferences, ensuring purified extracts for liquid chromatography-tandem mass spectrometry (LC-MS/MS) and other trace-level quantification workflows.

Technical Performance and Design

• Strong Anion Exchange (SAX) Chemistry: The quaternary amine ligand retains a permanent positive charge, enabling the capture and concentration of acidic target analytes regardless of the sample matrix pH.

• Targeted Matrix Wash: The ionic bond formed between the sorbent and the acidic analyte allows researchers to utilize organic wash solvents, selectively stripping away neutral lipids, proteins, and basic interferences.

• Fixed 96-Well Architecture: The standardized plate footprint interfaces with automated liquid handlers, vacuum manifolds, and positive pressure systems for high-throughput, parallel sample processing.

• Silica-Based Substrate: The 50 µm particle size and 60 Å pore diameter ensure reproducible flow rates and consistent lot-to-lot extraction recoveries for complex biological or environmental matrices.

Product Specifications

• Catalog Number: 500-0025-P01

• Product Line: ISOLUTE SAX

• Format: 96-Well Fixed Plate

• Sorbent Mass: 25 mg per well

• Retention Mechanism: Strong Anion Exchange (Quaternary Amine)

• Target Analytes: Acidic Compounds

Key Benefits & Applications

• Bioanalytical & Clinical Testing: Used for extracting acidic pharmaceutical drugs, endogenous acidic biomarkers, and metabolites from complex biological fluids like plasma, serum, or urine.

• Environmental & Food Safety: Effective for isolating and concentrating acidic pesticides, herbicides, or food additives from water samples and agricultural homogenates.

• Reduced Ion Suppression: Yields clean extracts by selectively excluding neutral and basic endogenous matrix components, directly increasing mass spectrometer signal intensity.

• High-Throughput Automation: The rigid well design maintains uniform flow rates across all 96 positions, preventing channeling or vacuum leaks during automated extraction protocols.

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