Corning 3540 Microplate 384 Well without Lid Black with Clear Bottom Case of 50

Corning 3540 384-Well Black/Clear Bottom Microplate (TC-Treated, with Lid)

The Corning 3540 is a high-density 384-well microplate engineered for sophisticated high-throughput screening (HTS) and cell-based assays. Designed specifically for fluorescence-based detection and high-content analysis, this plate features opaque black walls that effectively suppress well-to-well crosstalk and background auto-fluorescence. The optically clear, flat bottom provides a distortion-free window for bottom-reading plate readers and high-resolution inverted microscopy. Manufactured from medical-grade virgin polystyrene, the growth surface is vacuum-gas plasma treated (TC-treated) to ensure uniform cell attachment and expansion across all 384 wells. Supplied sterile and non-pyrogenic with a low-evaporation lid, the 3540 is optimized for miniaturized assays where data precision and reagent conservation are paramount.

Technical Performance and Design

• HTS-Optimized Geometry: The 384-well format allows for a 4-fold increase in data points per plate compared to standard 96-well formats, significantly reducing reagent consumption and processing time.

• Advanced Signal Control: Solid black pigmentation in the upper structure absorbs lateral light, ensuring that high-intensity signals from a single well do not interfere with neighboring low-signal wells.

• Superior Optical Clarity: The ultra-thin, clear polystyrene bottom is engineered for optimal light transmission, making it compatible with laser-based scanning systems and automated imaging platforms.

• Validated TC Surface: The hydrophilic surface modification promotes consistent cell distribution and robust anchorage, essential for maintainining monolayer integrity during automated washing or staining steps.

Product Specifications

• Catalog Number: 3540

• Format: 384-Well Microplate with Lid

• Color: Black Walls with Clear Bottom

• Surface Treatment: Tissue Culture (TC) Treated

• Well Volume: 112 µl (Total Capacity); 20–80 µl (Recommended Working Volume)

• Material: High-Clarity Polystyrene (PS)

• Sterility: Sterile (Gamma Irradiated); Non-pyrogenic

• Packaging: 20 Plates per Pack / 100 Plates per Case

Key Benefits & Applications

• High-Content Screening (HCS): Ideal for automated imaging and phenotypic assays where clear bottom access and opaque walls are required for high-resolution digital data acquisition.

• Fluorescence-Based Assays: Optimized for FRET, TR-FRET, and calcium flux assays (e.g., FLIPR®) where low background noise is critical for accurate signal quantification.

• Assay Miniaturization: The high-density well format enables researchers to scale down assay volumes, preserving expensive reagents and limited cell samples without sacrificing statistical power.

• Robotic Compatibility: Fully compliant with ANSI/SLAS standards for seamless integration with automated liquid handlers, plate stackers, and multi-mode detection systems.

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