{"product_id":"corning-46-055-cm-terrific-broth-1-l","title":"Corning Terrific Broth 1 L Case of 6 Bottles","description":"\u003ch3\u003eCorning 46-055-CM Terrific Broth Liquid Medium – 1 L Bottle\u003c\/h3\u003e\n\u003cp\u003eThe Corning 46-055-CM is a premium, ready-to-use 1 L liquid microbiological growth medium engineered explicitly for the high-density propagation and maintenance of recombinant \u003ci\u003eEscherichia coli\u003c\/i\u003e strains. Formulated under strict ISO 13485 quality standards, Terrific Broth (TB) is an enriched nutrient matrix containing higher concentrations of casein peptone and yeastolate than standard Luria-Bertani (LB) broths. It is supplemented with glycerol as a non-repressible carbohydrate carbon source and features an integrated potassium phosphate buffering system. This chemical architecture sustains prolonged bacterial logarithmic growth phases, yielding significantly higher cell masses and dramatically maximizing plasmid DNA or heterologous protein recovery during downstream nucleic acid purification workflows.\u003c\/p\u003e\n\n\u003ch3\u003eTechnical Performance and Design\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eEnriched Yeastolate \u0026amp; Peptone Matrix:\u003c\/b\u003e Formulated with 24.00 g\/L Yeastolate and 12.00 g\/L Casein Peptone. This high concentration of amino acids, vitamins, and trace elements supplies the structural baseline necessary to support highly dense bacterial biomasses.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eNon-Repressible Glycerol Carbon Source:\u003c\/b\u003e Utilizes 4.00 mL\/L Glycerol rather than glucose as the primary carbohydrate source. This prevents the rapid accumulation of acetic acid byproducts—a common cause of premature cell death and culture crash in glucose-supplemented mediums.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003ePhosphate Buffering Stabilization:\u003c\/b\u003e Incorporates a balanced mixture of $2.31\\text{ g\/L KH}_2\\text{PO}_4$ (monopotassium phosphate) and $12.54\\text{ g\/L K}_2\\text{HPO}_4$ (dipotassium phosphate). This system maintains physiological pH bounds against acid production, allowing cultures to thrive far beyond standard saturation thresholds.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eReady-to-Use (1X) Form:\u003c\/b\u003e Pre-mixed, sterile-filtered, and autoclaved inside a heavy-duty polystyrene storage bottle. This removes the lab variability, dust exposure, and preparation labor associated with compounding dehydrated powder media in-house.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eProduct Specifications\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eCatalog Number \/ MPN:\u003c\/b\u003e 46-055-CM (46055CM)\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eManufacturer \/ Brand:\u003c\/b\u003e Corning® \/ Mediatech\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eVolume Capacity:\u003c\/b\u003e 1 Liter (1 L)\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eMedia Type:\u003c\/b\u003e Terrific Broth (Liquid, 1X Form)\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eFormulation:\u003c\/b\u003e 12.00 g Casein Peptone, 24.00 g Yeastolate, 4.00 mL Glycerol, 2.31 g $\\text{KH}_2\\text{PO}_4$, 12.54 g $\\text{K}_2\\text{HPO}_4$ per Liter\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eStorage Profile:\u003c\/b\u003e Ambient storage between 15°C to 30°C\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eSterility Matrix:\u003c\/b\u003e Sterile-Filtered \u0026amp; Autoclavable, Lot-Validated Certificate of Analysis (CoA) Compliant\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eKey Benefits \u0026amp; Applications\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eMaximized Plasmid Yields:\u003c\/b\u003e The premier chosen liquid medium for expanding \u003ci\u003eE. coli\u003c\/i\u003e cultures in preparation for high-yield plasmid minipreps, midipreps, and maxipreps.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eRecombinant Protein Expression:\u003c\/b\u003e Supports massive target protein yields during IPTG- or auto-induction sequences, minimizing the total culture volumes required to collect adequate protein fractions.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eHigh Cell Density Cultivation:\u003c\/b\u003e Achieves up to a 4-to-10-fold increase in bacterial biomass and cell saturation profiles compared to basic Luria-Bertani (LB) broth formulations.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eValidated Manufacturing:\u003c\/b\u003e Produced within certified cGMP facilities, ensuring highly reproducible lot-to-lot chemical metrics for regulated academic and industrial biotechnology pipelines.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003chr\u003e\n\u003cp style=\"margin: 0; font-size: 0.8em;\"\u003e\u003ci\u003eAll product names, logos, and brands are property of their respective owners.\u003c\/i\u003e\u003c\/p\u003e","brand":"Corning","offers":[{"title":"Default Title","offer_id":44740522606844,"sku":"6444","price":75.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0627\/8463\/5132\/files\/corning-terrific-broth-1-l-case-of-6-bottles-other-corning-441218.jpg?v=1716058351","url":"https:\/\/lifesciencestrading.com\/products\/corning-46-055-cm-terrific-broth-1-l","provider":"Life Sciences Trading","version":"1.0","type":"link"}