Corning Culture Flask 75 cm2 Vent Cap U-Shaped 8 Flasks

Corning 3814 Cell Culture Flask – 75 cm², U-Shaped, Vent Cap

The Corning 3814 is a highly reliable 75 cm² cell culture flask engineered for the robust expansion of adherent mammalian cell lines. Constructed from premium, optically clear virgin polystyrene, it provides exceptional visibility for distortion-free cellular observation under an inverted microscope. A defining feature of this model is its U-shaped geometry; the rounded shoulders eliminate sharp corners, allowing for the unrestricted movement of cell scrapers and pipettes to ensure maximum sample recovery. Furthermore, the flask is equipped with a specialized vent cap containing a 0.2 µm hydrophobic filter. This allows for consistent, continuous gas exchange within a CO2 incubator while establishing an absolute barrier against microbial and fungal contamination. Pre-sterilized by gamma irradiation and certified non-pyrogenic, the 3814 is ready for immediate use in demanding tissue culture and virology workflows.

Technical Performance and Design

• U-Shaped Geometry: The rounded shoulders provide a highly ergonomic internal profile, preventing cell clumping in hard-to-reach corners and drastically improving the efficiency of trypsinization and mechanical scraping.

• 0.2 µm Vent Cap: The integrated hydrophobic membrane allows for vital gas exchange without the contamination risks associated with "cracking" or partially unscrewing standard solid caps.

• Tissue Culture Treated: The polystyrene surface undergoes a highly controlled proprietary treatment process that introduces hydrophilic groups, promoting the rapid and uniform attachment of anchorage-dependent cells.

• Stackable Profile: Designed with a flat top and a stabilizing base ridge, allowing multiple flasks to be securely stacked to maximize valuable shelf space inside crowded CO2 incubators.

Product Specifications

• Catalog Number: 3814

• Available Growth Area: 75 cm² (Standard T-75)

• Flask Shape: U-Shaped (Rounded Shoulders)

• Cap Style: Vent Cap with 0.2 µm Membrane

• Material: Optically Clear Polystyrene

• Sterility: Gamma Irradiated, Non-pyrogenic

Key Benefits & Applications

• Routine Cell Expansion: The industry-standard vessel size and surface treatment make it ideal for passaging common adherent lines such as HeLa, HEK293, and CHO cells.

• Maximized Cell Yield: The combination of the U-shaped design and wide neck access ensures that researchers can harvest nearly 100% of the confluent monolayer without leaving cells behind in crevices.

• Contamination Security: The vent cap eliminates the human error associated with manual cap venting, drastically reducing the chances of losing valuable cultures to airborne pathogens.

• Optical Clarity: The flawless virgin polystyrene allows for precise confluence checks and the early visual detection of morphological changes or contamination events.

Expiration: 2024-03-03.

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