Corning Culture Flask 3L Vented Cap Erlenmeyer Fernbach Sterile Case of 4

Corning 3L Polycarbonate Erlenmeyer Cell Culture Flask – Fernbach Design, Plain Flat Bottom, 0.2 µm Vented Cap, Optically Clear, Gamma Sterile (MPN: 431252)

Expiration: 2025-04-01.

The Corning Life Sciences 431252 (also cataloged as CLS431252 or 431252-CL) is a high-performance, single-use 3-liter bioprocess shaker flask engineered for high-yield cell culture expansion, media preparation, and recombinant protein expression. Manufactured from high-clarity, break-resistant virgin polycarbonate (USP Class VI), this flask features a classic wide-base Fernbach geometry designed to significantly increase liquid surface area and optimize gas exchange dynamics during orbital shaking. Outfitted with an orange polypropylene wide-mouth screw cap containing an integrated 0.2 µm hydrophobic membrane filter, the 431252 permits continuous, sterile gas exchange while providing an unyielding barrier against airborne micro-contaminants and fluid leakage. Supplied gamma-irradiated sterile and certified non-pyrogenic as well as RNase- and DNase-free, this 3000 mL vessel serves as an industry-standard scale-up link across biopharmaceutical development, cell therapy manufacturing, and fermentation cores.

Technical Performance and Structural Design

• Surface-Maximizing Fernbach Design: Engineered with a wide, squat Fernbach base footprint that dramatically increases the liquid-to-gas surface interface. This profile maximizes passive oxygenation rates and carbon dioxide stripping without requiring forced sparging systems.

• USP Class VI Polycarbonate Matrix: Injection-molded from medical-grade, shatterproof polycarbonate. This material provides crystal-clear optical transparency for rapid visual turbidity checks while withstanding structural stress during high-RPM orbital shaking routines.

• 0.2 µm Hydrophobic Membrane Vent Cap: Outfitted with a polypropylene threaded cap featuring an integrated 0.2 µm hydrophobic PTFE membrane. The microporous filter ensures continuous, sterile aeration during incubation loops without risking aerosolized media blowouts or environmental contamination.

• Plain Flat-Bottom Hydrodynamics: Features a smooth, non-baffled plain flat bottom. This non-baffled interior surface reduces fluid shear stress, making it the preferred choice for shear-sensitive mammalian cells, insect cell lines, and delicate suspension cultures.

Product Specifications

• Catalog Number / MPN: 431252 (Cross-listed as CLS431252 / 431252-CL)

• Manufacturer / Brand: Corning Life Sciences

• Nominal Working Volumetric Capacity: 3000 mL (3 Liters)

• Flask Geometry & Bottom Style: Conical Fernbach Design with a Plain Flat Bottom (Non-baffled format)

• Material Composition: Optical-Grade Polycarbonate (PC) Flask Body with Polypropylene (PP) Closure

• Cap Type & Color: Wide-Mouth Threaded Vent Cap with 0.2 µm Membrane (Orange)

• Overall Physical Exterior Dimensions: Approximately 9.06 inches Base Diameter x 9.92 inches Overall Height (230 mm Diameter x 252 mm Height)

• Biological Purity Status: Gamma Irradiated Sterile, Non-Pyrogenic, RNase-free, and DNase-free

Key Benefits and Applications

• Suspension Mammalian & Insect Cell Scale-Up: The standard baseline choice for scaling up CHO, HEK293, and Sf9/Sf21 suspension cell lines before inoculation into large seed-train bioreactors.

• Shear-Sensitive Cell Line Protection: The plain (non-baffled) bottom profile minimizes turbulent shear forces, protecting delicate cell membranes from mechanical lysis during continuous orbital shaking.

• Universal Orbital Shaker Compatibility: Drops smoothly into standard high-capacity 3L shaker clamps and sticky-mat platforms across major benchtop and incubator shakers.

• Aseptic Reagent & Buffer Holding: Excellent single-use sterile vessel choice for preparing, storing, and transferring sterile culture media, feed solutions, or intermediate process buffers.

All product names, logos, and brands are property of their respective owners.