Corning Culture Microplate 384 Well Black Low Volume Round Bottom Sealed Case of 50 Plates

Corning 384-Well Low Volume Solid Black Round Bottom NBS Microplates, Non-Sterile (4514) – Case of 50

The Corning 384-Well Low Volume Black Microplates with Nonbinding Surface (4514) are high-precision assay plates engineered for miniaturized fluorescence assays, high-throughput screening (HTS), and low-concentration compound/target binding studies. Constructed from opaque black, high-purity polystyrene, these microplates feature a light cone-shaped conical well geometry with round bottoms, designed to eliminate trapped air bubbles, lower working volume requirements, and reduce autofluorescence and signal crosstalk. Treated with Corning’s proprietary Nonbinding Surface (NBS) technology, the nonionic hydrophilic surface prevents non-specific protein, nucleic acid, and small-molecule adsorption to well walls. Supplied as a case of 50 non-sterile plates (packaged as 5 inner bags of 10 plates each, without lids), this consumable line delivers superior signal-to-noise performance for automated fluorometric and homogeneous assay workflows.

Technical Performance & Key Attributes

• Proprietary Nonbinding Surface (NBS) Treatment: Creates a nonionic hydrophilic surface (polyethylene oxide-like) that minimizes molecular interactions and prevents non-specific binding of proteins, DNA/RNA, and hydrophobic compounds at low concentrations.
• Miniaturized Low Volume Geometry: Conical light-cone well design with a round bottom allows a recommended working volume of just 5 µL to 40 µL, significantly reducing reagent consumption while maximizing signal stability.
• Solid Black Opaque Walls: Absorbs excitation light to minimize background autofluorescence and block well-to-well crosstalk during fluorometric and time-resolved fluorescence assays.
• Robotic Pipetting Efficiency: Features a square well top profile that increases the target opening area for automated liquid handling robotics and multi-channel dispensers.

Product Specifications

• Catalog Number / MPN: 4514 (Suggested replacement for Corning 3676)
• Primary Manufacturer: Corning Life Sciences
• Plate Format: 384-Well Low Volume Microplate (16 x 24 Array)
• Well Shape & Bottom Profile: Square Top with Round / Conical Bottom (U-Bottom)
• Total Well Volume: 50 µL per well
• Recommended Working Volume: 5 µL to 40 µL per well
• Material Composition: Optical-Grade Solid Black Polystyrene (PS)
• Surface Treatment Status: Nonbinding Surface (NBS) Treatment
• Sterility Status: Non-Sterile
• Lid Status: Supplied without lids
• Packaging Configuration: Case of 50 microplates (5 inner bags x 10 plates per bag)

Key Benefits & Applications

• Fluorescence Screening & HTS: Optimized for miniaturized fluorometric, TR-FRET, and homogeneous assay formats requiring minimal background interference and zero well-to-well crosstalk.
• Low Concentration Target Protection: NBS treatment prevents target biomolecules and small molecules from sticking to plastic walls, enhancing signal-to-noise ratios in delicate binding kinetics.
• Reagent Conservation: Low working volume range (5–40 µL) dramatically reduces enzyme, substrate, and fluorophore consumption during large-scale library screens.
• Automated Handling Integration: Conforms to standard ANSI/SLAS microplate dimensions for seamless integration into robotic grippers, plate stackers, and automated plate readers.

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