Corning Microplate 96 Well Black 50 Plates

Corning 3915 96-Well Black Flat Bottom Non-Binding Surface (NBS) Microplate

The Corning 3915 is a high-performance 96-well microplate engineered for the most demanding fluorescence-based assays and sensitive biochemical screens. Manufactured from black medical-grade polystyrene, this plate is designed to eliminate well-to-well crosstalk and significantly reduce background autodispersion. The proprietary Non-Binding Surface (NBS) treatment utilizes a non-ionic hydrophilic polyethylene oxide-like coating to prevent the attachment of proteins and nucleic acids at low concentrations. This combination makes the 3915 the industry standard for high-throughput screening (HTS), homogenous assays, and studies involving high-value or low-abundance biological molecules.

Technical Performance and Design

• Non-Binding Surface (NBS) Technology: The specialized coating creates a stable, hydrophilic barrier that minimizes non-specific binding of proteins and DNA, ensuring that nearly 100% of the analyte remains in solution for accurate quantification.

• Light-Absorbing Black Pigmentation: The solid black polystyrene construction absorbs light, preventing fluorescence "bleed-through" between adjacent wells and providing the lowest possible background for fluorescence-based detection.

• Precision Flat Bottom: The uniform, flat-bottom well geometry provides an ideal path for top-reading microplate readers and ensures consistent reagent distribution for homogeneous assays.

• Standardized ANSI/SLAS Footprint: Fully compatible with automated liquid handling workstations, robotic stackers, and high-speed plate readers used in drug discovery workflows.

Product Specifications

• Catalog Number: 3915

• Format: 96-Well Black Flat Bottom

• Surface Treatment: Non-Binding Surface (NBS)

• Total Well Volume: 360 µl

• Recommended Working Volume: 75 to 200 µl

• Sterility: Non-sterile (Standard for HTS biochemical assays)

• Packaging: 25 Plates per Bag / 100 Plates per Case

Key Benefits & Applications

• High-Throughput Screening (HTS): Ideal for enzymatic assays and ligand-binding studies where minimal protein loss and maximum signal-to-noise ratio are required.

• Fluorescence Polarization (FP): The black surface is specifically validated for FP assays, providing the high-sensitivity environment necessary to measure molecular interactions in solution.

• Low-Abundance Analyte Research: The NBS treatment prevents the "vanishing" of samples that typically occurs when low concentrations of proteins or peptides adhere to standard plastic surfaces.

• Consistent Data Integrity: Produced to rigorous quality standards to ensure well-to-well and plate-to-plate consistency, reducing the coefficient of variation (CV) in large-scale data sets.

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