Corning Microplate with Lid 96 Well 360 ul PS Sterile Indiv Sealed 24 Plates

Corning 3474 Costar Ultra-Low Attachment Microplate – 96-Well, 360 µL, Sterile

The Corning 3474 is a high-performance 96-well microplate engineered for applications requiring the total inhibition of cell attachment. Manufactured from high-clarity polystyrene, this plate features Corning’s proprietary Ultra-Low Attachment (ULA) surface—a covalently bound hydrogel layer that is hydrophylic, neutrally charged, and biologically inert. This surface effectively prevents the absorption of anchorage-dependent proteins, forcing cells to remain in a suspended state or to aggregate into uniform 3D spheroids. Featuring flat-bottom wells with a 360 µL capacity, the 3474 is ideal for studying stem cell differentiation, tumor biology, and embryoid body formation. Each plate is supplied sterile, individually sealed with a breathable lid to ensure optimal gas exchange while maintaining an aseptic environment. Certified non-pyrogenic and DNase/RNase-free, the 3474 provides the most reliable platform for reproducible 3D cell culture and suspension-based assays.

Technical Performance and Design

• Ultra-Low Attachment (ULA) Surface: The specialized hydrogel coating minimizes cell-to-surface interaction, ensuring that cells interact with each other rather than the plastic, which is critical for 3D spheroid formation.

• Optical Clarity: Constructed from premium polystyrene to ensure high light transmission and low background interference for microscopic observation and plate-reader detection.

• Standard SBS Footprint: Fully compliant with industry standards, allowing for seamless integration with automated liquid handlers, robotic incubators, and imaging systems.

• Breathable Lid Design: The included lid features a condensation ring and allows for uniform gas exchange across all 96 wells, reducing the "edge effect" during long-term incubation.

Product Specifications

• Catalog Number: 3474

• Well Count: 96 Wells

• Total Volume: 360 µL per well

• Material: Polystyrene with ULA Coating

• Well Shape: Flat Bottom

• Sterility: Sterile (Individually Wrapped)

Key Benefits & Applications

• Spheroid and Organoid Culture: The gold standard for oncology research, allowing for the consistent creation of multi-cellular tumor spheroids (MCTS) for drug sensitivity testing.

• Stem Cell Research: Prevents the premature differentiation of stem cells that can be triggered by surface attachment, maintaining pluripotency during expansion or embryoid body formation.

• Protein Adsorption Studies: The inert surface is ideal for assays where protein binding to the vessel wall must be strictly controlled to ensure accurate concentration measurements.

• High-Resolution Imaging: The flat-bottom geometry is optimized for inverted microscopy and high-content screening (HCS) platforms.

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