Cytiva GE Hollow Fiber Filter Tangential Flow Cartridge

Cytiva 56-4102-22 Hollow Fiber TFF Cartridge – CFP-1-E-4X2MA, 100K MWCO

The Cytiva 56-4102-22 is a precision-engineered hollow fiber tangential flow filtration (TFF) cartridge designed for the efficient concentration, clarification, and diafiltration of biological fluids. This CFP-1-E-4X2MA model features a high-performance Polysulfone (PS) membrane with a 100,000 Dalton (100K) Molecular Weight Cut-Off (MWCO), offering an ideal balance between rapid permeate flux and high product retention. The hollow fiber geometry provides a gentle, low-shear environment that is critical for maintaining the structural integrity of delicate proteins, monoclonal antibodies, and viral particles. Engineered for seamless integration into QuixStand and other benchtop TFF systems, this cartridge features a 1.0 mm fiber lumen diameter, making it particularly effective at handling feedstreams with moderate solids loading without significant fouling. Whether used for process development or small-scale clinical production, the 56-4102-22 ensures predictable linear scale-up and highly reproducible results.

Technical Performance and Design

• Polysulfone (PS) Membrane: Provides a robust, chemically resistant surface with low non-specific binding characteristics, ensuring maximum recovery of target biomolecules.

• 1.0 mm Fiber Lumen: The wider internal diameter of the fibers allows for the processing of viscous samples and cell lysates with a lower risk of lumen plugging compared to 0.5 mm alternatives.

• Tangential Flow Dynamics: The cross-flow action continuously sweeps the membrane surface, significantly reducing the "gel layer" formation and maintaining high filtration rates over extended run times.

• Sanitary Design: Constructed with biocompatible, USP Class VI compliant materials, designed to withstand standard sanitization protocols with mild sodium hydroxide (NaOH) solutions.

Product Specifications

• Catalog Number: 56-4102-22 (Model CFP-1-E-4X2MA)

• MWCO: 100,000 Daltons (100K)

• Membrane Material: Polysulfone (PS)

• Fiber ID: 1.0 mm

• Filtration Area: ~420 cm² (0.45 ft²)

• Housing Material: Polysulfone

Key Benefits & Applications

• Protein Concentration: Ideal for the rapid volume reduction of recombinant proteins and enzymes while maintaining high biological activity.

• Desalting & Diafiltration: Efficiently removes salts, solvents, and low-molecular-weight contaminants through continuous buffer exchange.

• Viral Vector Processing: The 100K MWCO is perfectly sized for the concentration of AAV and other viral particles while allowing smaller impurities to pass through the membrane.

• Cell Culture Harvesting: Used for the clarification of cell culture supernatants, providing a clean permeate for downstream chromatography steps.

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