Cytiva GE Hollow Fiber Filter Tangential Flow Cartridge 750k NMWC

Cytiva UFP-750-E-3MA MidGee™ Hollow Fiber TFF Cartridge (750 kDa, 110 cm²)

The Cytiva UFP-750-E-3MA is a laboratory-scale Tangential Flow Filtration (TFF) cartridge designed for the concentration, diafiltration, and fractionation of high-molecular-weight biological molecules. Featuring a 750 kDa Nominal Molecular Weight Cutoff (NMWC), this MidGee™ series cartridge is optimized for ultra-low volume applications, allowing for efficient processing of samples as small as 10 ml. The membrane is constructed from Low Adsorption Polyethersulfone (PES) to minimize fouling and non-specific binding, while the housing is made of durable Polysulfone (PS). This module is an essential tool for R&D laboratories focused on viral vector purification, vaccine development, and large protein complex research.

Technical Performance and Design

• 750 kDa Ultrafiltration Membrane: Specifically engineered to retain large viruses (such as Lentivirus and Adenovirus) and large protein aggregates while allowing smaller contaminating proteins, salts, and buffers to pass through to the permeate.

• Low Adsorption PES Media: The high-flux Polyethersulfone membrane provides a hydrophilic surface that reduces the adsorption of valuable biological analytes, ensuring high product recovery and stable flux rates.

• 1.0 mm "A" Lumen Diameter: The 1.0 mm internal fiber diameter provides a gentle, low-shear flow path that is ideal for sensitive biological structures and can handle moderately viscous feed streams without clogging.

• MidGee™ Small-Volume Efficiency: With a 110 cm² surface area, this cartridge is designed for "Size 3" systems, enabling high concentration factors in small-batch research and process development.

Product Specifications

• Catalog Number: UFP-750-E-3MA (56-4101-77)

• Cutoff (NMWC): 750,000 Daltons (750 kDa)

• Membrane Area: 110 cm²

• Lumen ID: 1.0 mm

• Materials: PES Membrane; Polysulfone (PS) Housing

• Connections: 1/2-inch TC (Process) and Hose Barb (Permeate)

• Sanitation: Autoclavable and compatible with standard Sodium Hydroxide (NaOH) cleaning protocols.

Key Benefits & Applications

• Viral Vector Concentration: The 750 kDa cutoff is the preferred choice for concentrating large viral particles with high yield and minimal shear-induced damage.

• Nanoparticle & Liposome Processing: Ideal for the washing and concentration of synthetic nanoparticles and lipid-based drug delivery systems.

• Fractionation of High-Value Proteins: Efficiently separates large complexes or IgM antibodies from smaller serum proteins and growth factors.

• Predictive Scalability: Part of the standardized Cytiva TFF family, allowing data generated on this MidGee™ scale to be directly applied to larger pilot and production-scale cartridges.

All product names, logos, and brands are property of their respective owners. MidGee is a trademark of Cytiva.